BACTE LAB FE Flashcards
This is the most commonly used stain for yeasts and molds.
Lactophenol Cotton Blue (LPCB)
Chloramphenicol is a broad spectrum antibiotic added to SDA in the culture of fungi in order to:
Inhibit bacterial growth
Pigmentation of fungi is due to the presence of what structures?
Spores
The initial hypha that develops from a conidium or spore is called:
Germ tube
What is the widely used method of enumerating or counting bacteria in meat and milk samples?
Total Plate Count (TPC)
This microorganism from undercooked hamburger meat can cause fatal kidney failure or hemolytic uremic syndrome.
E. coli
The two principal forms of fungi are the mold and the yeasts. The main element of the vegetative or growing form of the mold is known as what?
Hypha
Molds produce intertwined threads that form parts that arise above the surface and are referred to as the ___.
Aerial (hyphae)
The following fungi belong to phyla which do NOT produce sexual spores:
Deuteromycota and Chytridiomycota
The following are sexual spores produced by fungi EXCEPT:
Chlamydospore
What is the standard medium for culturing fungi in a laboratory setting?
Sabouraud Dextrose Agar (SDA)
What are the 2 main morphological forms of fungi?
Branching filaments and Unicellular yeasts
Among the given, which of the following are NOT asexual spores?
Ascospores and Basidiospores
This is added to the medium to inhibit the growth of nonpathogenic species of fungi.
Cyclohexidine
This reagent is added to clinical specimens to hydrolyze polysaccharides and leave hyphae intact.
KOH (Potassium Hydroxide)
What technique is used for rapid mounting and has a disadvantage of its transparency fading over time?
Adhesive tape method
A simple method that permits the growth of fungi gathered from the environment (in situ method.)
Slide culture technique
What is the term used to describe the septate hyphae of Zygomycetes members?
Coenocytic
A microorganism that is a common contaminant of hamburger meat.
E. coli
What medium is utilized for the total coliform test?
Brilliant Green Broth
True/False: Gas production with growth in EC broth in 24 ± 2 hours confirms fecal coliform presence.
True
The immediate source of most bacteria on carcasses after slaughter is the:
Hide and skins
Total plate count can be done in the following products EXCEPT:
Potable water
Spore-bearing septate hyphae form a brush and unbranched conidia chains from flask-shaped phialides. Which fungus is this?
Penicillium notatum
The following microorganisms are capnophiles EXCEPT:
Aspergillus niger
E. coli can adapt in intestinal and extraintestinal habitats because it is aerobic and can be anaerobic in its respiration?
True
The technique used in bacteriological analysis of wastewater is the ___ method.
Most Probable Number (MPN) or Multiple Tube Fermentation Technique (MTFT)
The usual asexual division in yeasts is by:
Formation of buds
Dermatophytes are pathogens causing what type of infection in animals and man?
Ringworm
The fungus Cryptococcus neoformans utilizes what substance present in the droppings of birds?
Creatinine
