Antibiotics/Antifungals/Antivirals Flashcards
Cell wall inhibitors
Penicillins
Cephalosporins
Carbapenems
Vancomycin
Bacitracin
Fosfomycin
Isoniazid
Acts on cell membrane
Polymyxins
Daptomycin
Antibiotics that inhibits DNA replication and transcription
Quinolones
Inhibits RNA polymerase
Rifampin
Acts on 50S subunit
Erythromycin
Clindamycin
Synercid
Pleuromutilins
Acts on 30S subunit
Aminoglycosides - gentamycin, streptomycin
Tetracycline
Glycylcyclines
Acts on both 30S and 50S
Linezolid
Acts on folic acid synthesis in cytoplasm
Sulfonamides
Trimethoprim
Mechanism of action of meropenem
Binds penicillin binding protein to inhibit cell wall synthesis
Mechanism of action of ciprofloxacin
Inhibits DNA topoisomerase
Mechanism of action of trimethoprim
Disruption of dihydrofolate reductase
Mechanism of action of erythromycin
Inhibition of translocation of tRNA at 50S ribosomal subunit
Mechanism of action of rifampicin
Inhibits DNA-dependent RNA-polymerase
Mechanism of action of colistin
Disruption of cell membrane via LPS and phospholipids
Mechanism of action of sulfamethoxazole
Inhibition of de novo folate biosynthesis
Mechanism of action of doxycycline
Competition with A site on 50S ribosomal subunit
Mechanism of resistance of VRE
Modification of drug target
van gene
Mechanism of aminoglycoside E. coli resistance
Enzymatic alterations of binding site
Mechanism of ESBL
Beta lactamases
Destruction of antibiotics
Mechanism of staph aureus with intermediate sensitivity to vancomycin
Global cell adaptation to antibiotic effects
Mechanism of action of entecavir
Guanosine analogue phosphorylated within virally infected cells into triphosphate that then incorporates into viral DNA - halts replication process
Mechanism of action of famciclovir
Guanosine derivative phosphorylated within virally infected cells to triphosphate that inhibits DNA polymerase
Mechanism of action of ritonavir
Inhibition of viral protease
Virus associated Kaposi’s sarcoma
HHV-8
Virus associated with Merkel cell carcinoma
Polyomavirus
Virus associated with primary CNS lymphoma
EBV
Virus associated with squamous cell anal cancer
HPV
Mechanism of action of echinocandins
Inhibits B-1, 3-D-glucan synthesis
Mechanism of action of polyenes (amphotericin B, nystatin)
Bind to membrane ergosterol, altering membrane integrity
Mechanism of imidazoles (ketoconazole) and triazoles (fluconazole, itraconazole)
Inhibits CYP450 enzyme lanosterol14 demethylase, results in decreased ergosterol production
Mechanism of action of terbinafine
Inhibits squalene epoxidase with resultant decrease in lanosterol and ergosterol production
Mechanism of action of griseofulvin
Inhibits fungal mitosis by binding to intracellular microtubular protein
Mechanism of action of 5-flucytosine
Converted to 5-fluorouracil, incorporated into fungal RNA inhibiting protein synthesis
Mechanism of action of B lactams
Binds to penicillin-binding-proteins –> decreases crosslinking of peptidoglycan layer
Examples of B lactam
Penicillin
Cephalosporins
Carbapenems
Monobactams
Generations of beta lactams
1st - cephalexin
2nd - cefaclor
3rd - cefixime
4th - cefepime
5th - ceftaroline
Mechanism of resistance for beta lactams
Cleavage of beta lactam ring by beta-lactamases
Mechanism of resistance for MRSA
PBP mutations
Mechanism of action for glycopeptides e.g. vancomycin, teicoplanin
Bind to D-alanyl-D-alanine section of peptidoglycan precursor –> inhibited peptidoglycan synthesis
Mechanism of resistance of vancomycin
Reduced penetration in gram negative bacteria
Change in peptidoglycan precursor structure
Mechanism of action for fosfomycin
Inactivate enolpyruvate transferase –> inhibition of N-acetylmuramic acid formation –> disruption of peptidoglycan synthesis
Adverse effects of carbapenems
Secondary fungal infections
CNS toxicity - highest risks of imipenem
GI upset
Rash Thrombophlebitis
Adverse effects of cephalosporins
Potential cross-reactivity in pts with penicillin allergies
AIHA
Vitamin K deficiency
Disulfiram-like reaction
Increases nephrotoxic effect of aminoglycosides
Neurotoxicity
Mechanism of action of aminoglycosides
Inhibits protein synthesis - 30S ribosomal subunit
Mechanism of resistance of aminoglycosides
Inactivating enzymes e.g. acetylation, phosphorylation, adenylation
Removal by efflux pumps
Mutation of bacterial ribosome binding site
Reduced penetrance
Anaerobic bacteria
Acidic environment
Mechanism of action of tetracycline
Inhibition of protein synthesis - 30S ribosomal subunit
Block incoming aminoacyl-tRNA with amino acids –> decrease protein synthesis
Mechanism of resistance of tetracycline
Reduced cell wall penetration
Removal by efflux pump
Production of protein that protects ribosome
Clinical use of daptomycin
Gram positive bacteria
S. aureus especially MRSA
VRE
Adverse effects of daptomycin
Reversible myopathy
Rhabdomyolysis
Allergic pneumonitis
Clinical use of colistin
Polymyxin
Severe gram negative bacteria that are multidrug resistant
Adverse effects of colistin
Nephrotoxicity
Neurotoxicity
Urticaria, eosinophilia and/or anaphylactoid reactions
Respiratory failure
Adverse effects of aminoglycosides
Nephrotoxicity
Ototoxicity and vestibulotoxicity
NM blockade
Teratogenecity
Contraindications of aminoglycoside
MG
Botulism
Pregnancy
Clinical use of tetracyclines
Bacteria that lack cell wall i.e. Mycoplasma
Intracelluar bacteria i.e. Rickettsia, Chlamydia, Anaplasma
Borrelia burgdorferi
Adverse effects of tetracyclines
Hepatotoxicity
Deposition in bones and teeth –> inhibition of bone growth and discolouration of teeth
Damage to mucous membranes i.e. oesophagitis
Photosensitivity
Fanconi syndrome
Pseudotumour cerebri
Mechanism of action of macrolides
Bind to 23S rRNA –> inhibition of transpeptidation, translocation and chain elongation –> decrease protein synthesis
Mechanism of resistance of macrolides
Reduced penetration
Efflux pumps
Methylation of 23S rRNA binding site –> inhibits binding of macrolides
Cross-resistance with clindamycin and streptogramins
Mutation of bacterial ribosome binding site
Mechanism of clindamycin
Impair transpeptidation –> inhibition of chain elongation –> decrease protein synthesis
Increase opsonisation and phagocytosis
Inhibits alpha toxin expression
Mechanism of resistance of clindamycin
Reduced penetration
Mutation of bacterial ribosome binding site
Mechanism of action of oxazolidinones i.e. linezolid
Prevents association of 50S with 30S subunit –> impairment of initiation complex formation –> early interruption of protein synthesis
Mechanism of resistance of oxazolidinones
Point mutation of 23S rRNA
Methylation of ribosomal subunit
Mechanism of action of chloramphenicol
Prevent binding of amino acid-containing aminoacyl-tRNA –> inhibition of peptidyltransferase –> reduce protein synthesis
Mechanism of resistance of chloramphenicol
Reduced penetration
Enzymatic inactivation by acetyltransferase (plasmid encoded)
Mechanism of action of fluoroquinolones i.e. ciprofloxacin, norfloxacin
Inhibit prokaryotic toposiomerase II (DNA gyrase) and topoisomerase IV –> inhibited DNA synthesis
Mechanism of resistance of fluoroquinolones
Mutations in DNA gyrase and topoisomerase IV
Reduced cell wall permeability
Efflux pumps (plasmid-encoded resistance)
Mechanism of action of metronidazole
Disruption of DNA integrity via free radical formation
Mechanism of resistance of metronidazole
Reduced activation due to decreased enzymatic activity
Mechanism of action of rifamycins (rifampin, rifabutin, rifaximin)
Blocks mRNA synthesis via inhibition of bacterial DNA-dependent RNA-polymerase –> reduce protein synthesis
Mechanism of resistance of rifamycins
Mutated RNA-polyermase –> reduce binding of rifamycins
Mechanism of action of isoniazid
Inhibits mycolic acid synthesis –> reduce cell wall synthesis
Mechanism of resistance of isoniazid
Mutation causing reduce KatG –> reduce expression of catalase-peroxidase
Mechanism of resistance of pyrazinamide
Mutations in RpsA gene coding for ribosomal protein S1
Mutation of action of ethambutol
Inhibits arabinosyltransferase –> reduce cell wall synthesis
Mechanism of resistance of ethambutol
Mutations in EmbCAB gene coding for arabinosyltransferase –> inability of drug to inhibit enzyme
Mechanism of action of dapsone
Competitive antagonism of para-aminobenzoic acid –> inhibited dihydrofolic acid synthesis
Mechanism of resistance of dapsone
Mutations in folP1 gene coding for dihydropteroate synthase –> reduced expression of dihydroteroate synthase
Mechanism of action of nitrofurantoin
Bind to bacterial ribosomes –> inhibition of DNA, RNA, and protein synthesis
Mechanism of resistance of nitrofurantoin
Enzyme-mediated reduction
Efflux pumps
Clinical use of tigecycline
Gram positive aerobes
MRSA
VRE
Anaerobes (broad spectrum)
Partially effective against gram-negative aerobes
Adverse effects of tigecycline
GI upset
Hepatotoxicity
Deposition in bones and teeth
Photosensitivity
Adverse effects of macrolides
Increased intestinal motility –> GI upset
QT interval prolongation
Acute cholestatic hepatitis
Eosinophilia
Rash
Increased risk of hypertrophic pylroic stenosis in infants up to 6 weeks og ageD
Drug reactions of macrolides
Enhances effect of oral anticoagulants
Erythromycin and clarithromycin - increased theophylline serum concentrations, CYP3A4 inhibition
Antibiotics associated with pseudomembranous colitis
Clindamycin
Fluoroquinolones
3rd and 4th generation cephalosporins
Adverse effects of clindamycin
GI upset
Pseudomembranous colitis
Fever
Teratogenicity
Adverse effects of oxazolidinones I.e. linezolid
GI upset
Pancytopenia due to BM suppression
Peripheral neuropathy
Serotonin syndrome - partially inhibits monoamine oxidase
Generations of fluoroquinolones
1st - nalidixic acid
2nd - norfloxacin, ciprofloxacin, ofloxacin
3rd - levofloxacin
4th - moxifloxacin, gemifloxacin
Adverse effects of fluoroquinolones
GI upset
Neurological symptoms - mild headache, dizziness, mood changes, peripheral neuropathy, lowers seizure threshold
Hyper/hypoglycaemia
QT prolongation
Photosensitivity
Skin rash
Superinfection
Muscle ache, leg cramps, tendinitis, tendon rupture
Adverse effects of metronidazole
Headache
Disulfiram-like reaction
Metallic taste
Peripheral neuropathy (prolonged used)
Vestibular dysfunction
Mechanism of action of diaminopyrimidine e.g. amifampridine
Inhibits dihydrofolate reductase
Uses NADPH to reduce dihydrofolate acid to tetrahydrofolic acid
Used to treat congenital myasthenia syndromes and LEMS
Mechanism of action of sulfonamides e.g. sulfomethoxazole
Inhibits dihydropteroate synthase
Adverse effects of sulfonamides
Drug interactions due to CYP450 inhibition
Displacement of other drugs from albumin
Nephrotoxicity esp. ATN
GI upset
Hyperkalaemia
Agranulocytosis
Aplastic anaemia, thrombocytopenia, pancytopenia
Haemolytic anaemia in G6PD-deficient patients
SJS
Hypersensitivity reactions
Photosensitivity
Fever
Adverse effects of diaminopyrimidine derivatives
Megaloblastic anaemia
Leukopenia, granulocytopenia
Hyperkalaemia
Increased creatinine - caused by decreased tubular secretion
Adverse effects of nitrofurantoin
Nitrofurantoin induced lung disease
Pulmonary fibrosis
Haemolytic anaemia
GI upset
Reversible peripheral neuropathy
Adverse effects of rifampicin
Red-orange discolouration of body fluids
Flu-like symptoms
Minor hepatotoxicity
CYP450 induction
False positive urine opiate screening
Adverse effects of isoniazid
Hepatotoxicty
Anion gap metabolic acidosis
Drug induced lupus erythematous
Benzo-refractory seizures
Vitamin B6 deficiency - peripheral neuropathy, sideroblastic anaemia, aplastic anaemia, thrombocytopenia, pellagra
Adverse effects of pyrazinamide
Hyperuricaemia
Hepatotoxicity
Adverse effects of ethambutol
Optic neuropathy
Hyperuricaemia
Adverse effects of dapsone
Methemoglobinemia
Haemolytic anaemia in G6Pd deficiency
Agranulocytosis
GI upset
Peripheral neuropathy
Mechanism of action of interferon alfa and -a
Antiviral and immunomodulatory effect via intercellular and intracellular mechanisms
Adverse effects of pegylated interferon alfa and -a
Flu like symptoms
BM suppression
CNS - depressive mood, seizures
Induction of autoantibodies
Myopathy
Mechanism of action of tenofovir
Nucleoside analogue - premature termination of DNA transcription via phosphorylation to triphosphate in hepatic cells
Adverse effects of tenofovir/adefovir
Nephrotoxicity
Headache and abdominal pain
Mechanism of action of entecavir, lamivudine, telbivudine
Nucleoside analogue - inhibition of reverse transcriptase
Adverse effects of entecavir, lamivudine, telbivudine
GI symptoms
Fever
Headache
Vasculitides
Neuropathies
Neutropenia
Lactic acidosis
Aciclovir, valaciclovir relationship
Valaciclovir is prodrug of aciclovir
Has greater oral bioavailability
Penciclovir, famciclovir relationship
Famciclovir is prodrug of penciclovir with greater bioavailability
Mechanism of action of aciclovir, valaciclovir, penciclovir, famciclovir
Guanosine analogue
Phosphylated drug is incorporated into replicating viral DNA strand –> inhibition of viral DNA polymerase via chain termination
Adverse reaction of aciclovir
Obstructive crystal-induced nephropathy
AKI
TTP
GI symptoms
Increases transminases
Mechanism of action of ganciclovir, valganciclovir
Guanosine analogue
Phosphorylation to 5’ monophosphate by CMV coded UL97 kinase –> further phosphorylation to triphosphate by cellular kinases
Inhibition of viral DNA polymerase –> termination of viral DNA synthesis
Adverse effects of ganciclovir, valganciclovir
Myelotoxicity
Nephrotoxicity
GI symptoms
CNS - headache, confusion, parasthesias
Mechanism of action of foscarnet
Pyrophosphate analogue
Adverse effects of foscarnet
Nephrotoxicity
GI symptoms
CNS - headache, confusion, seizures due to electrolyte abnormalities, parasthesias
Haemotological abnormalities - leukopenia, neutropenia
Mechanism of action of amantadine
M2 ion channel blocker
Weak NMDA receptor antagonist
Adverse effects of amantadine
Neurological - ataxia, anxiety
GI - nausea, vomiting
Livedo reticularis
Peripheral oedema
Mechanism of action of oseltamivir
Neuraminidase inhibitor
Adverse effects of oseltamivir
Pulmonary - URTI
GI - nausea, vomiting
Headache
Mechanism of action of ketoconazole
Inhibits 17a-hydroxylase/17,20-lyase
Adverse effects of amphotericin B
Arrhythmias
Nephrotoxicity
Fever, chills
IV phlebitis
Hypokalaemia, hypomagnesemia
Adverse effects of nystatin
GI symptoms
Contact dermatitis
SJS
Adverse effects of azoles
Hepatotoxicity
CYP450 inhibition
Local burning sensation
Gynaecomastia - ketoconazole
Adverse effects of terbinafine
Headache
Hepatotoxicity
Dysgeusia
GI upset
Adverse effects of echinocandins
Flushing
Hepatotoxicity
GI upset
Adverse effects of griseofulvin
Hepatotoxicity
Carcinogenic
Teratogenic
CYP450 induction
Confusion, headaches
Disulfarim-like reactions
Adverse effects of flucytosine
BM suppression
Hepatic injury
Renal failure
Which antibiotics do not cross the blood brain barrier?
Aminoglycosides
Erythromycin
Tetracyclines
Clindamycin
First generation cephalosporins
Carbapenems are inactive against which bacteria
MRSA
VRE
Enterococcus faecium
Mycoplasma
Chlamydia
Stenotrophonomas maltophilia