ANBI 470 Midterm Exam 1 Flashcards
1
Q
In Siri hybridization
A
Shows where on chromosome area of interest is located (uses fluorescence - FISH)
2
Q
4 examples of SNPs
A
1) PCR-RFLP
2) Sequencing
3) KASP
4) SNP array
3
Q
SNP array
A
Genotypes SNPs using microbeads + fluorescence
4
Q
Array CGH
A
Used detect insertions & deletions with oligonueotide probe + fluorescence
5
Q
4 examples of physical genome mapping
A
1) somatic cell
2) in situ
3) restriction
4) genome sequencing
6
Q
marker assisted selections advantages + disadvantages
A
Advantages: faster selection process + ideal for traits difficult to measure
Disadvantages: hitch hiking + need large population
7
Q
Micro satellite markers advantages & disadvantages
A
A: easy to PCR, easy to compare, multi allelic
D: not as abundant as SNPs and not causative mutation
