ACTIVITY 2 Flashcards
What are the 3 things that prokaryotes have that are not membrane bound?
(based from worksheet)
- genetic material
- respiratory apparatus
- photosynthetic pigments
4 basic characteristics of prokaryotes
- unicellular
- no organelles
- microscopic
- no nucleus
5 factors in DIFFERENTIATING prokaryotes
- morphology
- chemical composition
- nutritional requirements
- biochemical activities
- energy source
Mode of reproduction by prokaryotes
binary fission
What bacteria are known as PRODUCERS?
cyanobacteria
2 ways prokaryotes can be described
- cultural (macroscopic)
- microscopic
2 types of preparation under CULTURAL CHARACTERISTICS
plate and slant
CHARACTERISTICS need to be determined for the ff:
- Plate (4)
- Slant (3)
PLATE:
- color
- form
- margin
- elevation
SLANT:
- color
- form
- margin
What kind of agar to use to see the natural color of microorganisms?
nutrient agar
2 factors that affect the cultural characteristics of microorganisms
- components of medium
- incubation condition
What is a COLONY
- visible mass of microorganisms originated from a single cell
what macroscopic color are most microorganisms are?
and what shapes are typically gram + or gram - ?
non-chromogenic
gram - may appear cream, white, or gray (bacillus)
gram + may appear yellow (coccus)
FORM vs ELEVATION vs MARGIN
F: shape of colony
E: cross sectional shape of colony
M: shape of the edge of colony
FORMS on AGAR SLANT
uniform growth along line of inoculation
filiform
FORMS on AGAR SLANT
margins of growth have TOOTHED APPEARANCE
echinulate
FORMS on AGAR SLANT
separated colonies
beaded
FORMS on AGAR SLANT
thin, veil-like growth, and spreading
effuse
FORMS on AGAR SLANT
branched, tree-like growth
arborescent
FORMS on AGAR SLANT
root-like appearance
rhizoid
3 major shapes of bacteria
coccus
bacillus
spirillum
CULTURAL PLATE
12 forms
CULTURAL PLATE
8 elevations
CULTURAL PLATE
9 margins
MACROSCOPIC COLOR OF THE FOLLOWING:
- Micrococcus luteus
- Bacillus cereus
- Escherichia coli
- Serratia marcescens
- yellow
- white
- colorless
- red