8-OPPORTUNISTIC FUNGI Flashcards
MOT:
inhalation of conidia
Form colonies within
several days
soil and decaying matter
Saprophytic fungi
Disease caused:
Phaehyphomycosis
Hyalohyphomycosis
Inhibited by many
antimicrobial agents
Must be repeatedly (?) in patients
isolated
Hyphae are
hyaline
Conidiogenous cells
Phialide
Annellides
Determinate: stops elongating when 1st conidium appears
Phialide
Indeterminate: continues to grow even when 1st conidium appears
Annellides
arises from pores of the hyphae
Poroconidia
glabrous/ velvety
DEMATIACEOUS
dark brown to black septate hyphae
DEMATIACEOUS
asexual reproduction
DEMATIACEOUS
causes Aspergillosis
Aspergillus Group
conidiophores terminate in a swollen vesicle
Aspergillus Group
Bronchopulmonary Disease: Farmer’s Lung
Aspergillus Group
Swimmer’s Ear
Aspergillus Group
- produces one series of structures between vesicle and conidium
Uniseriate
- forms two series of intermediate structures
Biseriate
- HYALINE SEPTATE
- Aspergillus Group
- Penicillus Group
conidiophores branching into a penicillus
Penicillus Group
brush-like arrangement due to branching of conidiophore
Penicillus Group
Cottony
ZYGOMYCETES/ ASEPTATE
fast growers: 4 or 3 to 5 days
ZYGOMYCETES/ ASEPTATE
Sexual/ asexual (sporangiospore)
ZYGOMYCETES/ ASEPTATE
few or no septum
ZYGOMYCETES/ ASEPTATE
misdiagnosed as bacterial sinusitis
Alternaria
easiest to identify among all fungi
Curvularia
only cause chronic sinusitis on immunocompetent px
Curvularia
Phaeohyphomycosis
Alternaria
sinusitis, asthma, osteomyelitis, eye and ear infection
Alternaria
respiratory infections, meningistis, osteomyelitis
Bipolaris
allergies, onychomycosis, endocarditis
Curvularia
mycotic keratitis, allergies
Drechslera
shades of brown to black
Alternaria
cottony and dirty gray to black
Curvularia
Poroconidia: club shaped
Alternaria
Poroconidia: aranged acropetally
Alternaria
with transverse and longitudinal septa
Alternaria
Conidiophores: geniculate (bent knee)
Bipolaris
Conidia: sympodial and indeterminate
Bipolaris
Conidia: pea pod shaped
Bipolaris
Hyphae: dematiaceous septate with thin walls
Conidia: with transverse septum shaped like ellipse
3 to 5 cells
Curvularia
“crescent roll”
Curvularia
Conidiophores: single, branched and geniculate
Drechslera
Conidia: sympodial indeterminate
Drechslera
skin allergies
Epicoccum
Hyphae: intertwined and branched together
Epicoccum
Conidiophore: short
Epicoccum
Conidia: globose to subglobose
Epicoccum
clinically significant
Fusarium
can be isolated
Fusarium
most famous opportunistic fungi
Fusarium
infected contact lenses; affected 100 individuals
Fusarium
keratitis, onychomycosis, fungemia (blood)
Fusarium
endocarditis, keratitis
Helminthosporium
keratitis
Nigrospora
Rose to mauve to purple to yellow
Fusarium
Hyphae: intertwined and branched together
Epicoccum
Conidiophore: short Conidia: globose to subglobose
Epicoccum
no conidiophores
Fusarium
abundant macroconidia with fewer microconidia
Fusarium
Conidiophore: determinate, straight
Helminthosporium
Conidia: slightly curved with a tapering end
Helminthosporium
Conidia: dark and oval (most distinct appearance)
Nigrospora
attached to the conidiophore at its side
Nigrospora
kidney-like arrangement
Nigrospora
colonization, allergy, disseminated infection, toxicity
Aspergillus fumigatus
due to toxin production
Aspergillus flavus
otomycosis, aspergilloma, nasal sinus infection
Aspergillus niger
invasive disseminated aspergillosis meningitis, osteomyelitis, endocarditis
Aspergillus terreus
smallest Asperigilli
Aspergillus terreus
SABHI: granular/fluffy or powdery growth within 2 days or (3-6 days: flat white colonies)
Aspergillus fumigatus
Culture Media: Modified SDA with chloramphenicol or gentamicin
Aspergillus fumigatus
w/ Abs: to inhibit bacte growth
Culture Media
Czapek’s Agar (mostly used)
Aspergillus fumigatus
with magnesium and ferrous sulfate with sucrose and potassium salts to enhance pigmentation
Czapek’s Agar
w/ Incubator at 500oC
Czapek’s Agar
grows at 500 C
Czapek’s Agar
usually a Hospital Acquired Infection
OPPORTUNISTIC FUNGI
affects immunosuppressed patients (ex. AIDS, HIV, diabetes)
OPPORTUNISTIC FUNGI
rapid growers
OPPORTUNISTIC FUNGI
Alternaria
Fusarium
Significant
OPPORTUNISTIC FUNGI
Can be contaminants
OPPORTUNISTIC FUNGI
Used to confirm diagnosis
OPPORTUNISTIC FUNGI
Microscopic identification (not limited to macroscopic or colonies)
OPPORTUNISTIC FUNGI
(dematiacious fungi; involves skin and subcutaneous)
Phaehyphomycosis
(forms hyphae)
Hyalohyphomycosis
- where conidia arises
Conidiogenous cells
: produces conidia
asexual reproduction
treatment: antibiotic
Alternaria
seen in grasses/leaves
Alternaria
seen in grass/leaves, decaying vegetations
Curvularia
with tail
Drechslera
growth upwards/youngest cell is at the tip
Alternaria
pea pod shaped
Bipolaris
clinically significant
Fusarium
can be isolated
Fusarium
most famous opportunistic fungi
Fusarium
Culture: grows at 370 C
Aspergillus flavus
SDA: 3 – 6 days; yellow to yellowgreen pigment
Aspergillus flavus
Culture: grows at 25 – 370 C
Aspergillus niger
SDA: 2 – 6 days; flat and white with jet black conidia
Aspergillus niger
Resembles sprinkled “black pepper” (same w/ P. notatum)
SDA
Culture: 25 – 300 C
Aspergillus terreus
SDA: 5 – 10 days; heavy sporulation
Aspergillus terreus
Hyphae: broad, branches at 450
Aspergillus fumigatus
Conidiophores: smooth walled, light green or brown
Aspergillus fumigatus
with foot cell
Aspergillus fumigatus
Vesicles: flask shaped, uniseriate flaskshaped phialides
Aspergillus fumigatus
Phialoconidia: round and echinulate
Aspergillus fumigatus
Stain: LCPB
Aspergillus fumigatus
looks like a child who stuck his finger in a live socket
Aspergillus fumigatus
Hyphae: broad, with foot cell
Aspergillus flavus
Conidiophores: thick-walled, coarsely roughened
Aspergillus flavus
Vesicles: large and globose, biseriate
Aspergillus flavus
Phialoconodia: unicellular and globose
Aspergillus flavus
Hyphae: similar to others
Aspergillus niger
Conidiophores: same with A. fumigatus, longest
Aspergillus niger
Vesicles: spherical, biseriate, jetblack
Aspergillus niger
Phialides: flask-shaped
Aspergillus niger
Phialoconidia: produced basipetally
Aspergillus niger
same with A. flavus
Aspergillus terreus
Vesicle: dome shaped, biseriate
Aspergillus terreus
Cause: Chronic Fungal Sinusitis
Penicillium
Seen everywhere
Penicillium
Confused as Penicillium
Paecilomyces
rarely cause infections
Penicillium
contaminant in hospitals, pulmonary infections
Paecilomyces
contaminant, onychomycosis
Scopulariopsis
usually 4 days (rapid growers)
Penicillium
except Penicillium marneffei, 2 weeks
Penicillium
velvety to powdery
Penicillium
colonies: blue-green with white reverse
Penicillium
3 to 4 days (rapid growers)
Paecilomyces
Flat, granular to velvety colonies in shades of tan, brownish gold, or mauve
Paecilomyces
o Serious care must be taken
Paecilomyces
o BSC w/ heap filter
Paecilomyces
2 – 4 days
Scopulariopsis
velvety to powdery
Scopulariopsis
Hyphae: thin Conidiophores: erect, penicillus, with secondary phialide
Penicillium
Phialoconidia: basipetal growth
Penicillium
Hyphae: hyaline septate
Paecilomyces
Conidiophores: erect with irregular branching
Paecilomyces
o with metullae and phialides
Paecilomyces
o phialides have long tapering necks
Paecilomyces
Phialoconidia: produced at the tips
Paecilomyces
Hyphae: broad
Scopulariopsis
Annellophores: short septate
Scopulariopsis
o cannot be distinguished from hyphae
Scopulariopsis
o resembles Penicillium
Scopulariopsis
Annellides: cylindrical or flask-shaped
Scopulariopsis
Annelloconidia: basipetal growth, pyriform (pearshaped) - with nipple-like projections
Scopulariopsis
Contaminant (hospital)
Acremonium
Trichoderma
Hyalohyphomycosis
CM: pulmonary and skin infections
Trichoderma
non-pathogenic
Gliocladium
Sepedonium
Recovered in the environment worldwide
Trichoderma
waxy, velvety with pink reverse
Acremonium
4 – 5 days covering the plate with flat white colonies growth
Trichoderma
Hyphae: delicate and thin forming intertwining ropes
Acremonium
Phialophores: slender, upright or at right angle; long and tapering at the tip
Acremonium
Phialoconidia: unicellular, egg-shaped in clusters
Acremonium
Hyphae: thin
Trichoderma
Phialophores: short and erect, branching at right angle; swells at the center and tapers at the tip
Trichoderma
Phialoconidia: subglobose to elliptical in clusters
Trichoderma
Conidia: green
Trichoderma
Rarely causes disease
Chrysosporium
Recovered in nails
Chrysosporium
adiaspiromycosis
hyalohyphomycosis
Chrysosporium
(enlargement of conidia in tisue without replication)
adiaspiromycosis
6 days, floccose and flat + thick or gray or tan pigment
Chrysosporium
Hyphae: with spiral vegetative structures
Chrysosporium
Conidiophores: similar to hyphae
Chrysosporium
Conidia: one-celled, clavate; with numerous arthroconidia
Chrysosporium
Mucor + Rhizopus
Rhizomucor
Most common-causing human disease
Rhizopus
Patients w/ diabetic and ketoacidosis
Rhizopus
May also be fatal to healthy patients
Rhizopus
Rarely causes human disease
Syncephalastrum
Cutaneous infections
Syncephalastrum
Resembles Aspergillus
Syncephalastrum
Rhinocerebralzygomycoses
Mucor
Young: Cottony, dirty white colonies
Mucor
Mature: Mousy brown to gray
Mucor
salt and pepper colony surface (covers the entire agar)
Rhizopus
woolly, cottony
Rhizopus
Young: white
Mature: gray to brown
Rhizopus
Resembles a test tube which contains a stack of marbles inside
Syncephalastrum
Columellae: cone-shaped with a pointed apex; resembles a Hershey’s Kiss
Absidia
Rhizoid: rudimentary
Absidia
Rhizomucor
Syncephalastrum
Rhizoids: well developed
Rhizopus
Rhizoids: none
Mucor
Sporangiophore: long and straight, with collarette
Mucor
Columellae: varying shapes
Mucor
Sporangium: globose
Mucor
Hyphae: widest hyphae among others
Rhizomucor
Sporangiophores: arise irregularly, intermodal; with collarette; without apophysis
Rhizomucor
Columellae: globose
Rhizomucor
Sporangium: round and black
Rhizomucor
Columellae: hemispherical with a flattened base; when mature, it bends resembling an up-side-down umbrella
Rhizopus
Merosporangium: resembles tubes or fingers that radiate from the around the vesicle
Syncephalastrum
Hyphae: thin walled; branching at right angles
Absidia
Sporangiophore: 2- 5 internodal group; apophysis remains when sporangium ruptures; with collarette
Absidia
Sporangium: pyriform, becomes brown or gray with age
Absidia
Hyphae: thick walled
Mucor
Hyphae: thin walled
Rhizopus
Sporangiophores: light brown, long and straight; nodal; with apophysis
Rhizopus
Sporangium: with varying size and with flattened base
Rhizopus
Hyphae: not as wide as other
Syncephalastrum
Zygomycetes: branches at right angles
Syncephalastrum
Sporangiophores: arise sympodially; long and erect; branches are short and curved; enlarges to a swollen vesicle
Syncephalastrum
Hershey’s
Absidia
Internodal
Absidia
Rhizomucor
Rudimentary
Absidia
Rhizomucor
Syncephalastrum
Arched stolon
Absidia
Variable
Mucor
Solitary
Mucor
None
Mucor
No apophysis
Mucor
Globose
Rhizomucor
Wide hyphae
Rhizomucor
Hemispherical
Rhizopus
Nodal
Rhizopus
Well developed
Rhizopus
Columella collapses
Rhizopus
Round vesicle
Syncephalastrum
Straight, branching
Syncephalastrum
Merosporangia
Syncephalastrum