8-OPPORTUNISTIC FUNGI Flashcards
MOT:
inhalation of conidia
Form colonies within
several days
soil and decaying matter
Saprophytic fungi
Disease caused:
Phaehyphomycosis
Hyalohyphomycosis
Inhibited by many
antimicrobial agents
Must be repeatedly (?) in patients
isolated
Hyphae are
hyaline
Conidiogenous cells
Phialide
Annellides
Determinate: stops elongating when 1st conidium appears
Phialide
Indeterminate: continues to grow even when 1st conidium appears
Annellides
arises from pores of the hyphae
Poroconidia
glabrous/ velvety
DEMATIACEOUS
dark brown to black septate hyphae
DEMATIACEOUS
asexual reproduction
DEMATIACEOUS
causes Aspergillosis
Aspergillus Group
conidiophores terminate in a swollen vesicle
Aspergillus Group
Bronchopulmonary Disease: Farmer’s Lung
Aspergillus Group
Swimmer’s Ear
Aspergillus Group
- produces one series of structures between vesicle and conidium
Uniseriate
- forms two series of intermediate structures
Biseriate
- HYALINE SEPTATE
- Aspergillus Group
- Penicillus Group
conidiophores branching into a penicillus
Penicillus Group
brush-like arrangement due to branching of conidiophore
Penicillus Group
Cottony
ZYGOMYCETES/ ASEPTATE
fast growers: 4 or 3 to 5 days
ZYGOMYCETES/ ASEPTATE
Sexual/ asexual (sporangiospore)
ZYGOMYCETES/ ASEPTATE
few or no septum
ZYGOMYCETES/ ASEPTATE
misdiagnosed as bacterial sinusitis
Alternaria
easiest to identify among all fungi
Curvularia
only cause chronic sinusitis on immunocompetent px
Curvularia
Phaeohyphomycosis
Alternaria
sinusitis, asthma, osteomyelitis, eye and ear infection
Alternaria
respiratory infections, meningistis, osteomyelitis
Bipolaris
allergies, onychomycosis, endocarditis
Curvularia
mycotic keratitis, allergies
Drechslera
shades of brown to black
Alternaria
cottony and dirty gray to black
Curvularia
Poroconidia: club shaped
Alternaria
Poroconidia: aranged acropetally
Alternaria
with transverse and longitudinal septa
Alternaria
Conidiophores: geniculate (bent knee)
Bipolaris
Conidia: sympodial and indeterminate
Bipolaris
Conidia: pea pod shaped
Bipolaris
Hyphae: dematiaceous septate with thin walls
Conidia: with transverse septum shaped like ellipse
3 to 5 cells
Curvularia
“crescent roll”
Curvularia
Conidiophores: single, branched and geniculate
Drechslera
Conidia: sympodial indeterminate
Drechslera
skin allergies
Epicoccum
Hyphae: intertwined and branched together
Epicoccum
Conidiophore: short
Epicoccum
Conidia: globose to subglobose
Epicoccum
clinically significant
Fusarium
can be isolated
Fusarium
most famous opportunistic fungi
Fusarium
infected contact lenses; affected 100 individuals
Fusarium
keratitis, onychomycosis, fungemia (blood)
Fusarium
endocarditis, keratitis
Helminthosporium
keratitis
Nigrospora
Rose to mauve to purple to yellow
Fusarium
Hyphae: intertwined and branched together
Epicoccum
Conidiophore: short Conidia: globose to subglobose
Epicoccum
no conidiophores
Fusarium
abundant macroconidia with fewer microconidia
Fusarium
Conidiophore: determinate, straight
Helminthosporium
Conidia: slightly curved with a tapering end
Helminthosporium
Conidia: dark and oval (most distinct appearance)
Nigrospora
attached to the conidiophore at its side
Nigrospora
kidney-like arrangement
Nigrospora
colonization, allergy, disseminated infection, toxicity
Aspergillus fumigatus
due to toxin production
Aspergillus flavus
otomycosis, aspergilloma, nasal sinus infection
Aspergillus niger
invasive disseminated aspergillosis meningitis, osteomyelitis, endocarditis
Aspergillus terreus
smallest Asperigilli
Aspergillus terreus
SABHI: granular/fluffy or powdery growth within 2 days or (3-6 days: flat white colonies)
Aspergillus fumigatus
Culture Media: Modified SDA with chloramphenicol or gentamicin
Aspergillus fumigatus
w/ Abs: to inhibit bacte growth
Culture Media
Czapek’s Agar (mostly used)
Aspergillus fumigatus
with magnesium and ferrous sulfate with sucrose and potassium salts to enhance pigmentation
Czapek’s Agar
w/ Incubator at 500oC
Czapek’s Agar
grows at 500 C
Czapek’s Agar
usually a Hospital Acquired Infection
OPPORTUNISTIC FUNGI
affects immunosuppressed patients (ex. AIDS, HIV, diabetes)
OPPORTUNISTIC FUNGI
rapid growers
OPPORTUNISTIC FUNGI
Alternaria
Fusarium
Significant
OPPORTUNISTIC FUNGI
Can be contaminants
OPPORTUNISTIC FUNGI
Used to confirm diagnosis
OPPORTUNISTIC FUNGI
Microscopic identification (not limited to macroscopic or colonies)
OPPORTUNISTIC FUNGI
(dematiacious fungi; involves skin and subcutaneous)
Phaehyphomycosis
(forms hyphae)
Hyalohyphomycosis
- where conidia arises
Conidiogenous cells
: produces conidia
asexual reproduction