6.4.2: Proteins Flashcards
Proteins
Condensation polymers of amino acids which are joined by peptide links.
Two amino acids form a
with a … link
dipeptide
with a peptide link
Peptide link
C(=O)-N(-H)
If the two amino acids forming a dipeptide are different…
then two different dipeptides can be formed,
because the amino acids can join either way round
Method for hydrolysing proteins
aqueous 6 moldm-3 hydrochloric acid
and then heat the mixture under reflux for 24hrs
Four stages of protein structure
primary
secondary
tertiary
quaternary
Primary structure
The sequence of amino acids in the polypeptide chain
Secondary structure
Peptide links between amino acids form hydrogen bonds with each other, causing the chain to spiral into an alpha helix or fold into a beta pleated sheet.
Tertiary structure
The chain of amino acids becomes coiled and folded in a way that identifies the protein and forms a 3D structure. Extra bonds such as disulfide and hydrogen bonds form in different parts of the polypeptide chain.
Two types of bonds that hold proteins in shape
hydrogen bonds
disulfide bonds
Residue
An amino acid that is part of a protein
Disulfide bonds
Occurs between residues of the amino acid cysteine
to form -S-S-
Disulfide bonds in proteins
help to stabilise the tertiary structure
Factors that affect hydrogen and disulfide bonds so can change the shape of proteins
2
temperature and pH