46 Virology 4 Flashcards
T or F: RNA is more stable than DNA.
Very false
How should viruses be preserved for laboratory testing?
Shielded from light and refrigerated or frozen
Which came first, canine or feline parvovirus?
Feline
How did parvovirus jump from cats to dogs?
Mutations in the parvovirus capsid to allow binding to canine transferrin receptors
Describe how a viral antigen is isolated.
Sample is added to a tube with liquid and filtered. Then nuclease is added to chop up free nucleic acid to avoid confounding the rxn. Chemical agent is added to break open capsid and extract viral DNA into the liquid. Viral DNA is filtered and purified.
Describe how DNA is sequenced.
Nucleic acids are placed in a tube with PCR primer, DNA polymerase, nucleotides, and labeled nucleotides. The labeled nucleotides stop the strand from growing, so the end result is all different lengths of DNA strands with different colored labels at the end. These are then run through capillary gel via electrophoresis and interpreted by a computer.
What is differential PCR?
Sequence specific probes that selectively detect live attenuated vaccine vs. naturally acquired infection.
T or F: RNA dependent RNA polymerase cannot error correct.
T
T or F: RNA viruses are highly mutable.
T
What is the advantage of being a highly mutable RNA virus?
It allows the virus to adapt and explode in the environment
The smaller the genome, the (more/less) mutational tolerance.
More
What birds are particularly susceptible to WNV?
American crows and ravens
