2.17 Translation Flashcards
What are the three termination codons
UGA
URG
URA
What is the translation Initiation codon
AUG (Methionine)
Describe the structure of a tRNA molecule
Clover leaf structure as a result of intramolecule H bonding
70-80 nucleotides long
CCA at 3’ terminus
What part of tRNA recognizes mRNA
The anitcodon
What enzyme binds tRNAs with their appropriate amino acid/
Aminoacyl tRNA Synthetase
A ribosome has two subunits. What are they and what is the overall Ribosome structure called?
Ribosome 60s and 40s
Make Ribosome 80s
What are the three phases of translation?
Initiation
Elongation
Termination
What two things compose the Initiator complex during the initiation phase?
- Methionyl-initiator tRNA
2. 40s subunit of Ribosome
What recognizes Met-tRNA and binds it to 40s ribosomal subunit?
Eukaryotic Initiation factor 2 (eIF2)
Initiation in eukaryotes requires at least 10 proteins designated as what?
eIF’s
The 40s ribosomal unit travels down message until it reaches what codon?
AUG
What are the three tRNA-binding sites
P (Peptidyl)
A (Aminoacyl)
E (Exit)
What brings the second aminoacyl tRNA to the A site
EF-tu coupled to GTP
GTP is used to catalyze reaction
What is the role of EF-T?
It replaces the GDP with a GTP in the EF-Tu complex. This allows for a renewal of use
What is the difference between EF-Tu and eIF-2
EF-Tu is the elongation factor
eIF-2 is the initiating factor
-Both use GTP
