2012 biochem midterm Flashcards
Which Histone is NOT present in the nucleosome core?
a. H1
b. H2
c. H3
d. H4
e. H5
a. H1
We have 1 primer amplified within 20 cycles of PCR techniques. How many folds do we have?
a. 20 folds
b. 200 folds
c. 2,000 folds
d. 20,000 folds
e. 200,000 folds
a. 20 folds
What process will be affected if a mutation occurs in the census sequence AAUAAA?
a. 5’ Capping
b. Addition of 3’ Poly-A-tail
c. Splicing
d. Removal of Introns
e. RNA Initiation
b. Addition of 3’ Poly-A-tail
Taking gene from one person & incorporating it with another person. What is it called?
a. Recombinant DNA
b. Restriction modification
c. DNA mutation
d. DNA signaling
a. Recombinant DNA
What’s the property of Collagen? a. Hypo-methylated b. Hyper-methylated C. Contain 2 identical polypeptide chain D. Heavily glycosylated E. Stabilized by hydrophobic interactions
D. Heavily glycosylated
Why’s the reaction stopped when we used ddNTP?
a. 3’-OH is used
b. 3’-H is used
c. Primer is hydrolyzed
d. The annealing temperature is too high
b. 3’-H is used
What disturbs the secondary structure of the protein?
a. Lysine
b. Isolysine
c. Proline
d. Glycine
c. Proline
Gum Bleeding is caused by a deficiency of Vitamin C. What biochemical change will occur?
a. A decrease in the hydroxylation of Proline
b. A decrease in the hydroxylation of Lysine
c. A decrease in the hydroxylation of Glycine
a. A decrease in the hydroxylation of Proline
Lack of which OH prevent ddNTP?
a. 1’-OH
b. 2’-OH
c. 3’-OH
d. 4’-OH
e. 5’-OH
c. 3’-OH
What helps insert a protein with M6P in Golgi?
a. Lysosomes
b. Cytoplasm
c. Membrane
d. ER
e. Receptor
a. Lysosomes
Aminoacyl tRNA (aa-tRNA) that carry amino acid to translation are highly reactive. What factor stabilizes them in the cytoplasm?
a. EFG
b. EF-1
c. EF-3
d. Tu
e. Ts
d. Tu
How do suicide enzymes affect substrate?
a. Bind to allosteric site
b. Bind to active site
c. Disrupt synthesis
d. Disrupt degradation
b. Bind to active site
What stimulates glycogen synthesis in muscles?
a. Ca
b. Epinephrine
c. Norepinephrine
d. Insulin
e. Glucogon
d. Insulin
What’s the key feature in prion structures?
a. Change in primary protein
b. Excessive beta sheets
c. Increase in alpha-helix
d. Decrease in proline
b. Excessive beta sheets
What’s the property of highly substrate active sites?
a. High Km
b. Vm
c. Sigmoid-shaped
c. Sigmoid-shaped
Who has the highest % of having CF?
a. White
b. Hispanic
c. Black
d. Asian
a. White
During protein translation, an antibiotic analog aa-tRNA occupies the P-site. What’s inhibited?
a. Elongation
b. Initiation
c. Termination
d. Translocation
b. Initiation
A gene coding for lysosomal proteins is missing leader sequence. Where will the protein go?
a. Cytoplasm
b. Secretory
c. Mitochondria
d. Nucleus
e. Lysosome
e. Lysosome
What’s the indication of the change of β-Hemoglobin to sickle cell anemia?
Mutation of one AA
What kind of mutation does the gene encoding phenylalanine hydroxylase enzyme have?
a. Deletion
b. Splice
c. Nonsense
d. Insertion
e. Missense
e. Missense
What’s the characteristic of CREB?
Transcription Factor
Which enzyme activity signals Energy cell deficiency?
a. Na/K ATPase
b. hexokinase
c. AMPase
d. Glycogen synthesis
e. Protein Kinase A
a. Na/K ATPase
What’s the type of inheritance of CF?
a. X-linked Recessive
b. Autosomal Dominant
c. Autosomal Recessive
d. X-linked Dominant
c. Autosomal Recessive
What is the characteristic of metaphasic chromosome?
a. Naked DNA molecule w/o any histones
b. Single Stranded DNA Molecule
c. A DNA molecule that is synthesized at the G1 stage of cell cycle
d. 4 strands of DNA with histones
e. 1 strand of DNA with histones
d. 4 strands of DNA with histones
What’s the characteristic of HK (housekeeping gene)?
a. Continuously expressed
b. Regulated
c. Highly methylated Poorly acetylated
d. Highly phosphorylated
a. Continuously expressed
In CF, What’s the % of the affected offspring if the 2 parents were carriers?
a. 0
b. 25
c. 50
d. 75
e. 100
b. 25
Which DNA sequence is used for DNA fingerprinting?
a. Microsatellite DNA
b. Promoter region
c. Hypervariable minisatellite
d. Telomeric DNA Coding DNA
c. Hypervariable minisatellite
Which of the following is a characteristic of actively transcribed gene?
a. Found in euchromatic
b. Insensitive to α-amantin
c. Transcribed DNA Polymerase
d. Poorly acetylated
a. Found in euchromatic
What general characteristic is shared by most highly regulated intracellular enzymes?
a. Catalyze fastest step in the pathway
b. Rapidly degraded in the cytosol
c. Can be applied to michaelis-menten equation
d. Unchanged by phosphorylation
b. Rapidly degraded in the cytosol
What’s the function of a restriction enzyme?
a. Prevent formation of sugar-phosphate backbone
b. Breakdown DNA & RNA into nucleotides
c. Prevent formation of chemical bonds between pairs of nucleotides
d. Synthesis of DNA during replication
b. Breakdown of DNA molecules between specific nucleotides
b. Breakdown of DNA molecules between specific nucleotides
Phenylketonuria is what type of inborn error of metabolism?
a. Amino acid
b. Lipids
c. Carbohydrates
d. Purine
e. Pyrimidine
a. Amino acid
What is the characteristic of Transcription initiation factor?
a. Cis-acting
b. Trans-acting
c. Regulating mRNA splicing
d. Multibinding sites
e. Forming RNA-protein complexes
b. Trans-acting
What is necessary for cooperativity in enzymes?
a. Multiple substrate binding sites
b. Chaperone proteins
c. Allosteric binding sites
a. Multiple substrate binding sites
What’s the main function of DNA Polymerase?
a. Add nucleotides to free 3’OH end of DNA
b. Act as helicase enzyme
c. Involves in DNA Repair
a. Add nucleotides to free 3’OH end of DNA
What’s a characteristic of Proto-Oncogenes?
a. activated genes
b. cancer-causing genes
c. cellular gene that has the potential to become cancerous
c. cellular gene that has the potential to become cancerous
What’s the primer of glycogen synthesis?
Glycogenin
What’s the role of nucleotide analogs in cancer treatment?
a. Inhibit protein synthesis
b. Inhibit DNA Synthesis
c. Inhibit cell growth
b. Inhibit DNA Synthesis
