[13] Microscopy Techniques Flashcards
What topics are covered in the “Microscopy Techniques” deck?
- Fluorescence microscopy
- Confocal microscopy
- Electron microscopy
Define ‘Fluorescence microscopy’
A microscopy technique that uses fluorescence to generate an image
Define ‘Confocal microscopy’
A type of fluorescence microscopy with superior resolution and depth selectivity
Define ‘Electron microscopy’
A microscopy technique that uses a beam of electrons to create an image
How does ‘Fluorescence microscopy’ work?
It illuminates the specimen with light of a certain wavelength and then images the fluorescence that is emitted
How does ‘Confocal microscopy’ work?
It uses a spatial pinhole to eliminate out-of-focus light in specimens that are thicker than the focal plane
How does ‘Electron microscopy’ work?
It uses a beam of electrons instead of light to magnify the specimen up to two million times its actual size
What is ‘resolution’ in microscopy?
The ability to distinguish two points as separate in an image
What does ‘fluorescence’ mean in the context of microscopy?
The emission of light by a substance that has absorbed light or other electromagnetic radiation
What is the advantage of ‘Confocal microscopy’ over ‘Fluorescence microscopy’?
It allows for more precise imaging of thick specimens by removing out-of-focus light
What is ‘immunofluorescence’ in microscopy?
A technique that uses antibodies labeled with fluorophores to detect specific targets
What is the function of ‘antibodies’ in ‘immunofluorescence’?
To bind to and mark specific targets for detection
What is the main advantage of ‘Electron microscopy’ over other microscopy techniques?
It provides much higher resolution, allowing detailed views of structures at the cellular and molecular level
What is ‘Scanning Electron Microscopy (SEM)’?
A type of electron microscopy that images the sample surface by scanning it with a high-energy beam of electrons
Define ‘SEM’
Scanning Electron Microscopy
