[05] Molecular Biology Techniques Flashcards
What is the subject of the “Molecular Biology Techniques” deck?
- PCR
- Electrophoresis
- Blotting techniques
- Gene editing techniques (like CRISPR)
- Sequencing
Define ‘PCR’ in the context of Molecular Biology
A method used to rapidly make millions to billions of copies of a specific DNA sample
What is ‘Electrophoresis’ in Molecular Biology?
A method for separation and analysis of macromolecules and their fragments
Explain ‘Blotting techniques’ in Molecular Biology
Methods that transfer DNA, RNA, and protein onto a carrier so they can be stained and examined
Define ‘CRISPR’ in the context of Molecular Biology
A gene editing technology that can selectively alter DNA sequences and modify gene function
What is ‘Sequencing’ in Molecular Biology?
The process of determining the nucleic acid sequence – the order of nucleotides in DNA
What is the full form of ‘PCR’?
Polymerase Chain Reaction
What are the main steps in a PCR process?
- Denaturation
- Annealing
- Extension
Define ‘Denaturation’ in the context of PCR
The step where the double strand DNA molecule is heated to separate it into two single strands
Define ‘Annealing’ in the context of PCR
The step where the temperature is lowered to enable the DNA primers to attach to the template DNA
Define ‘Extension’ in the context of PCR
The step where a new strand of DNA is made by the Taq polymerase enzyme
What is the purpose of ‘Electrophoresis’?
To separate DNA, RNA, or protein molecules based on their size and electrical charge
Define ‘Southern Blotting’
A method used to check for the presence of a specific DNA sequence in a DNA sample
Define ‘Northern Blotting’
A technique used to detect specific RNA molecules among a mixture of RNA
Define ‘Western Blotting’
An analytical technique used to detect specific proteins in a sample
