[10] Cell Culture Techniques Flashcards
What is the subject of the “Cell Culture Techniques” deck?
- Cell lines
- Culturing techniques
- Contamination prevention
- Assays
Define ‘Cell lines’ in the context of Cell Culture Techniques
Cells that can be continuously cultured in vitro and have a uniform genetic makeup
What are ‘Culturing techniques’ in Cell Culture Techniques?
Methods used to grow and maintain cells under controlled conditions
Explain ‘Contamination prevention’ in Cell Culture Techniques
Steps taken to avoid the introduction of foreign materials or organisms into cell cultures
Define ‘Assays’ in the context of Cell Culture Techniques
Analytical procedures to assess the presence, amount, or functional activity of a target entity
What is a ‘Primary cell line’?
Cells taken directly from a living organism and processed to grow in culture
What is a ‘Continuous cell line’?
Cells that have acquired the ability to proliferate indefinitely
What is ‘Subculturing’ or ‘Passaging’?
The process of removing the cells from a culture and transferring them into a new vessel
What is the purpose of ‘Serum’ in cell culture?
To provide nutrients, hormones, growth factors and proteins necessary for cell growth
What is ‘Aseptic technique’?
A set of practices aimed at preventing contamination in cell cultures
List some common sources of contamination in cell culture
- Operator
- Culture medium
- Equipment
- Air
How is contamination prevented in cell culture?
- Sterilizing all equipment and surfaces
- Using aseptic technique
- Regularly monitoring cultures
Define ‘Cell viability assays’
Techniques to determine the proportion of live, healthy cells within a population
What is ‘MTT assay’?
A colorimetric assay for assessing cell metabolic activity
What is the purpose of ‘Cell proliferation assays’?
To measure the ability of cells to divide and grow
