[11] Protein Analysis Techniques Flashcards
What topics are covered in the “Protein Analysis Techniques” deck?
- Protein extraction
- Protein purification
- SDS-PAGE
- Western blotting
- Mass spectrometry
Define ‘Protein extraction’
The process of isolating proteins from the complex mixture in a cell or tissue
What is ‘Protein purification’?
A series of processes intended to isolate a single type of protein from a complex mixture
Explain ‘SDS-PAGE’
Sodium dodecyl sulfate polyacrylamide gel electrophoresis, a technique used to separate proteins based on their molecular weight
What is ‘Western blotting’?
A technique for detecting specific protein molecules from among a mixture of proteins
What is ‘Mass spectrometry’?
An analytical technique to determine the mass, structure and chemical properties of molecules
What is the purpose of ‘Protein extraction’?
To separate proteins from a cell or tissue for further analysis
Describe the steps involved in ‘Protein purification’
- Cell lysis
- Centrifugation
- Precipitation and solubilization
- Dialysis
Define ‘SDS’ in SDS-PAGE
Sodium dodecyl sulfate
Define ‘PAGE’ in SDS-PAGE
Polyacrylamide gel electrophoresis
How does ‘Western blotting’ work?
It transfers proteins from the SDS-PAGE gel to a membrane, followed by detection using specific antibodies
What is the primary use of ‘Mass spectrometry’ in protein analysis?
It is used to identify proteins and characterize their structure
What are some methods of ‘Protein extraction’?
- Mechanical disruption
- Detergents
- Organic solvents
- Enzymes
What is the objective of ‘Cell lysis’ in protein purification?
To break the cell membrane and release the proteins inside
What is the role of ‘Centrifugation’ in protein purification?
To separate proteins based on their size and density
