WNT signalling and exosomes

Question 1

What is Wnt?

Answer 1

used for cell-communication during embryogenesis

Question 2

What is the four step mechanism for Wnt

Answer 2

1. Bind to GPCRs on cell surface\
2. Relay of signal through cytoplasm
3. B-catenin is released from DC
4. B-catenin forms complex with TCF in nucleus to turn on Wnt target gene

Question 3

In what type of exosomes are Wnt signals found?

Answer 3

Exosomes with TSG101

Question 4

What is the role of Evi

Answer 4

Is a Wnt cargo receptor that releases Wnt from exosomes

Question 5

What is the role of the retromer complex?

Answer 5

• Important to sort membrane proteins and recycle trans memebrane receptors from
  endosomes to golgi
• Acts upstream of MVB sorting

Question 6

What retromer is associated with signalling for Evi-Wnt loading into exosomes?

Answer 6

Retromer SNX3