Non-coding RNAs

Question 1

Define a non-coding RNA

Answer 1

mRNAs that do not code for proteins but instead perform functions as mRNA molecules

Question 2

What are the positions of the target on a mRNA?

Answer 2

bp positions 2-8

Question 3

Drosha is responsible for cleaving what type of microRNA, and what length does it cut it into?

Answer 3

• Primary microRNA

- 65 nt step loop precursor miRNA

Question 4

What is the essential cofactor for DROSHA ?

Answer 4

DGCR8: it maintains the primary miRNA in the correct conformation

Question 5

What type of miRNA does DICER cut into a duplex?

Answer 5

pre-miRNA

Question 6

Define RISC

Answer 6

• RNA induced silencing complex

- completes the final step by isolating the guide strand for miRNA activity

Question 7

What is the normal length of miRNAs?

Answer 7

22 nt

Question 8

What are processing bodies?

Answer 8

o Provide a place for microRNA to function
o Regions of the ER that accumulate mRNA and silence it
o A gel like substance, it is involved in degrading mRNA