Exosomes and Metastasis

Question 1

Metastasis definition

Answer 1

the development of secondary malignant growths at a distance from a primary site of cancer.

Question 2

What are some effects cancerous EVs could have on the micro environment?

Answer 2

o Persistent migration: use of EVs to pave the way that the cell moves
o Formation and stabilization: EVs remodel surrounding tissue to allow cancer cell anchorage
o ECM remodeling: EVs will disrupt ECM to facilitate passage of cancer cell and pass along resistance

Question 3

What is the role of EGFR in metastasis

Answer 3

It invades the membrane of liver cells and represses miR26a/b

Question 4

What is the role of HGF?

Answer 4

As Hepatocyte growth factor, it is linked to cancer and tumor development

Question 5

What is HGFs receptor, and what role does it play?

Answer 5

C-met: widely expressed in different forms of cancer

Question 6

What pathway is HGF - cMet pathway associated with?

Answer 6

Proliferation, angiogenesis

Question 7

Of the two, HGF or cMET, which is more highly expressed in liver metasteses?

Answer 7

cMET

Question 8

What miR does EGFR target in order to activate HGF?

Answer 8

miR 26a/b

Question 9

What is the best place for a miR to bind for inhibiton of target?

Answer 9

3’UTR