Exosomes

Question 1

Definition of exosomes

Answer 1

Subset of EVs with unique markers that are 40-100 nm in size

Question 2

What are four proteins specifically enriched in exosomes?

Answer 2

Alix, flotillin, TSG101, CD63/81

Question 3

What is the role of Alix and TSG101 proteins

Answer 3

Involved in MVB biogenesis

Question 4

What is the role of flotillin?

Answer 4

membrane transport and fusion

Question 5

What is the role of proteins like CD63?

Answer 5

Integrins

Question 6

What is an ILV?

Answer 6

intraluminal vesicles. They are inside MVBs, and are where exosomes arise from

Question 7

What is the role of the RAB family

Answer 7

Control different steps of trafficking

Question 8

Role of transmembrane domain proteins

Answer 8

Select cargoes for exosome excretion

Question 9

Role of NSF attachment protein receptor (SNARE)

Answer 9

Instrumental in the fusion of the lipid bilayers of exosome and target cell after docking

Question 10

What does ESCRT stand for

Answer 10

Endosomal Sorting Complexes Required for Transport

- Allow unique membrane remodeling that results in MVB and budding

Question 11

What are three ways to determine where exosomes go?

Answer 11

o Flourescently stain donor cells exosomes
o Put Luc reporter on membrane surface of donor cell, exosomes made from it, go to recipient cell
o Cre recombinase
 Exosomes contain mRNA that contains reporter gene

Question 12

Heat shock protiens

Answer 12

HSC70, 90