Techniques Flashcards
1
Q
Immunoprecipitation
A
- It is the technique of precipitating a protein antigen out of solution by means of an antibody that specifically binds to that particular protein
- This process can used to separate and isolate a protein out of a solution containing thousands of proteins
- This technique was used in the YAP/TAZ paper to precipitate out these proteins, and see what was attached to YAP and TAZ
2
Q
Western Blotting
A
- A technique that separates a protein mixture based on the size of the polypeptide
- Makes use of antibodies to highlight the protein of interest
3
Q
Genetic reporting
A
- A reporter gene is a gene that researchers attach to a regulatory sequence of another gene of interest in bacteria, cell culture, etc
- Reporters have the same promoters as a target TF
- Used to measure activity of a transcription factor
4
Q
Immunofluorescence
A
- Use of a primary antibody to find protein antigen: secondary antibody conjugated to immunofluorescence
- We can observe the activity of specific proteins in the context of a cell
5
Q
siRNA knockout
A
- The technique by which the RNA for a certain protein is not expressed, leading to inhibited function that protein is responsible for
6
Q
Electron microscopy
A
- Way to visualize the shape of sub-cellular structures like proteins
7
Q
Transposon flanked expression cassettes
A
The way that they viewed the SHH going up the filopodia
