MicroRNA targets and identification

Question 1

Define bioinformatics

Answer 1

o Allow us to predict mRNA targets for microRNAs

Question 2

Name two methods of transcriptomics for identifying mRNA targets

Answer 2

1. mRNA microarrays

2. next generation sequencing

Question 3

What is the function of CLIP sequencing?

Answer 3

• Explores specificity and range of miRNA action in vivo

- CLIP identifies mRNA target sites for individual RNA binding proteins and miRNA