Wet Mount Slide Technique Flashcards
What is a wet mount slide?
A wet mount slide is a preparation technique used in microscopy to observe live specimens in a liquid medium.
True or False: Wet mount slides can only be used for dead specimens.
False
What type of medium is typically used in a wet mount slide?
Liquid medium, often water or saline.
Fill in the blank: A wet mount slide allows for the observation of __________ in their natural state.
live organisms
Which equipment is essential for preparing a wet mount slide?
A microscope and a glass slide.
What is the purpose of adding a coverslip in a wet mount slide?
To flatten the specimen and prevent contamination.
Multiple choice: What type of specimens can be observed using a wet mount slide? A) Bacteria B) Algae C) Protozoa D) All of the above
D) All of the above
What is the main advantage of using a wet mount slide?
It allows for the observation of living cells and their behavior.
True or False: Wet mount slides are suitable for observing thick or opaque specimens.
False
What is the first step in preparing a wet mount slide?
Place a drop of liquid medium on the center of a clean glass slide.
Fill in the blank: The __________ is used to secure the coverslip and prevent air bubbles.
angle technique
Multiple choice: Which of the following is NOT a common liquid medium for wet mounts? A) Water B) Oil C) Saline D) Glycerin
B) Oil
What type of microscope is typically used for viewing wet mount slides?
Light microscope.
True or False: Wet mount slides can be stored for long periods of time.
False
What is a common application of wet mount slides in microbiology?
To observe motility of microorganisms.
Fill in the blank: Wet mount slides are often used in __________ to study living cells.
laboratories
What should be done if air bubbles are present in a wet mount slide?
They should be removed by gently tapping the coverslip.
Multiple choice: Which of the following is a disadvantage of wet mount slides? A) Difficult to prepare B) Cannot observe live specimens C) Limited observation time D) Requires expensive equipment
C) Limited observation time
What is the role of staining in wet mount slides?
To enhance visibility of specific structures within the specimen.
True or False: Coverslips can be reused for wet mount slides.
False
What is the recommended thickness of the liquid layer in a wet mount slide?
As thin as possible to allow light to pass through.
Fill in the blank: Wet mount slides are often used in __________ studies to observe cellular processes.
biological
What is the significance of using a clean slide when preparing a wet mount?
To avoid contamination and ensure accurate observations.
Multiple choice: When should you adjust the focus while viewing a wet mount slide? A) Before placing the coverslip B) After placing the coverslip C) Only at low magnification D) Only at high magnification
B) After placing the coverslip
What can be observed in a wet mount slide of pond water?
Various microorganisms such as protozoa and algae.
True or False: Wet mount slides can be used to observe cellular division.
True
What is a wet mount slide?
A simple technique used in microscopy to observe live or liquid-based specimens.
What materials are needed to prepare a wet mount slide?
Microscope slide, coverslip, dropper or pipette, specimen (e.g., pond water, cells, bacteria, or tissue sample), distilled water or stain (e.g., methylene blue or iodine, if needed).
What is the first step in preparing a wet mount slide?
Place a small drop of water or the liquid sample in the center of the microscope slide.
What should you do if the specimen is solid?
Place the solid specimen in the water droplet.
When should you add a stain?
Add a drop of stain like methylene blue if the specimen is transparent to enhance contrast.
How should you lower the coverslip?
Hold the coverslip at a 45-degree angle and slowly lower it onto the water drop to avoid air bubbles.
What should you do if there is excess liquid?
Use a paper towel at the edge of the coverslip to absorb excess liquid.
What is the final step in examining the slide?
Start with the lowest magnification and adjust focus as needed.
What is the purpose of preparing a wet mount slide?
It is useful for observing live microorganisms, cells, and small structures in their natural state.
