Observing Bacteria Flashcards
What is the primary method for observing bacteria?
Microscopy
True or False: Bacteria can be observed with the naked eye.
False
Fill in the blank: The most common type of microscope used to observe bacteria is the ______ microscope.
light
What is the function of a staining technique in observing bacteria?
To enhance contrast and visibility of bacterial cells.
Name one common stain used for bacterial observation.
Gram stain
What does the Gram stain differentiate between?
Gram-positive and Gram-negative bacteria
Multiple Choice: Which of the following is NOT a type of microscopy used for observing bacteria? A) Electron microscopy B) Fluorescence microscopy C) Infrared microscopy
C) Infrared microscopy
Short Answer: What is the purpose of using a phase-contrast microscope?
To observe live cells without staining.
True or False: Bacteria can be cultured on solid media for observation.
True
What is the role of agar in bacterial culture media?
To provide a solid surface for bacterial growth.
Fill in the blank: Bacteria that require oxygen for growth are called ______ bacteria.
aerobic
What is a colony in the context of bacteria?
A visible mass of bacteria originating from a single cell.
Multiple Choice: Which microscopy technique allows for the visualization of live cells in real time? A) Scanning electron microscopy B) Light microscopy C) Transmission electron microscopy
B) Light microscopy
True or False: All bacteria are harmful to humans.
False
What is the purpose of using a biosafety cabinet when observing pathogenic bacteria?
To protect the user and the environment from exposure.
Short Answer: What is the significance of motility in bacterial observation?
It helps identify bacterial species and their behavior.
Fill in the blank: Bacteria that do not require oxygen are called ______ bacteria.
anaerobic
What is the use of an oil immersion lens in microscopy?
To increase resolution and clarity of the image.
Multiple Choice: Which stain is used to visualize bacterial capsules? A) Gram stain B) Capsule stain C) Acid-fast stain
B) Capsule stain
True or False: Bacteria can be identified by their shape.
True
Short Answer: What are the three basic shapes of bacteria?
Cocci, bacilli, and spirilla.
Fill in the blank: Bacteria that are spherical in shape are called ______.
cocci
What is the purpose of a selective medium in bacterial culture?
To inhibit the growth of certain bacteria while allowing others to grow.
Multiple Choice: Which type of microscopy provides the highest resolution? A) Light microscopy B) Scanning electron microscopy C) Transmission electron microscopy
C) Transmission electron microscopy
True or False: Staining is not necessary for observing bacteria under a microscope.
True
Short Answer: What is the principle behind fluorescence microscopy?
It uses fluorescent dyes to label specific cellular components.
Fill in the blank: The process of spreading a bacterial sample on a slide is called ______.
smearing
