Intro To Microscope

Question 1

What is a microscope?

Answer 1

A microscope is an instrument used to see objects that are too small for the naked eye.

Question 2

True or False: A microscope can only magnify images.

Answer 2

False

Question 3

What are the two main types of microscopes?

Answer 3

Light microscopes and electron microscopes.

Question 4

Fill in the blank: The _______ lens is the lens closest to the specimen in a microscope.

Answer 4

objective

Question 5

What is the function of the eyepiece in a microscope?

Answer 5

The eyepiece magnifies the image produced by the objective lens.

Question 6

What is the term for the ability of a microscope to distinguish two close objects as separate?

Answer 6

Resolution

Question 7

Multiple Choice: Which type of microscope uses beams of electrons for imaging?

Answer 7

Electron microscope

Question 8

What is the typical magnification range of a light microscope?

Answer 8

10x to 1000x

Question 9

True or False: The higher the magnification, the lower the resolution.

Answer 9

True

Question 10

Fill in the blank: The part of the microscope that controls the amount of light reaching the specimen is called the _______.

Answer 10

diaphragm

Question 11

What is the primary use of a compound microscope?

Answer 11

To view small specimens and thin sections of materials.

Question 12

Multiple Choice: Which part of the microscope holds the objective lenses?

Answer 12

Nosepiece

Question 13

What is the purpose of the stage on a microscope?

Answer 13

To hold the specimen in place for viewing.

Question 14

True or False: All microscopes require a light source.

Answer 14

False

Question 15

What type of microscopy uses fluorescent markers?

Answer 15

Fluorescence microscopy

Question 16

Fill in the blank: The _______ is used to focus the image in a microscope.

Answer 16

fine adjustment knob

Question 17

What is the main advantage of an electron microscope over a light microscope?

Answer 17

Higher resolution and magnification.

Question 18

Multiple Choice: What is the maximum magnification of a typical light microscope?

Answer 18

1000x

Question 19

What is the role of immersion oil in microscopy?

Answer 19

To increase the resolution by reducing light refraction.

Question 20

True or False: Microscopes can only be used for biological specimens.

Answer 20

False

Question 21

What is the term for the distance between the objective lens and the specimen in a microscope?

Answer 21

Working distance

Question 22

Fill in the blank: The _______ lens in a microscope is responsible for the initial magnification.

Answer 22

objective

Question 23

What is the main limitation of a light microscope?

Answer 23

Limited resolution compared to electron microscopes.

Question 24

Multiple Choice: Which technique allows for viewing live cells under a microscope?

Answer 24

Phase contrast microscopy

Question 25

What is the purpose of staining specimens before viewing them under a microscope?

Answer 25

To enhance contrast and visibility of structures.

Question 26

True or False: The ocular lens usually has a magnification of 10x.

Answer 26

True

Question 27

What is the first step in using a light microscope?

Answer 27

Carry your microscope to your work station using both hands

Question 28

What should be done to the condenser before using the microscope?

Answer 28

Raise the condenser to its maximum position nearly even with the stage

Question 29

What is the recommended first objective to use when observing a specimen?

Answer 29

Low power objective (usually 4X)

Question 30

What adjustment should be made for a binocular microscope?

Answer 30

Adjust the position of the two oculars to match your own interpupillary distance

Question 31

Which knob is used first to bring the image into focus?

Answer 31

Coarse focus adjustment knob

Question 32

After using the coarse focus adjustment knob, what should be used for sharp focus?

Answer 32

Fine focus adjustment knob

Question 33

What should be adjusted to produce optimum illumination and contrast?

Answer 33

Iris diaphragm and condenser position

Question 34

What is the procedure for observing a nonbacterial specimen?

Answer 34

Progress through the objectives until you see the degree of structural detail necessary

Question 35

What must be done before advancing to the next objective?

Answer 35

Position a desirable portion of the specimen in the center of the field

Question 36

What should be done with freshly-prepared slides after use?

Answer 36

Dispose of them in a jar of disinfectant

Question 37

What is the last step when finished using the microscope for the day?

Answer 37

Return the microscope to its appropriate storage place

Question 38

Fill in the blank: You should clean any oil off the lenses using _______.

Answer 38

lens paper

Question 39

True or False: The light intensity should be raised to maximum before turning off the light.

Answer 39

False

Question 40

What should be done with the electrical cord after using the microscope?

Answer 40

Wrap the electrical cord around the base of the microscope

Question 41

What should be done to the mechanical stage at the end of the session?

Answer 41

Center the mechanical stage

Question 42

What is a key consideration when scanning the specimen for details?

Answer 42

Scan to locate a promising region to examine in more detail