Simple Stain Flashcards

1
Q

What is a simple stain?

A

A simple stain is a basic staining technique used to highlight the morphology and arrangement of bacteria.

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2
Q

True or False: Simple stains can differentiate between different types of bacteria.

A

False

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3
Q

What is the primary purpose of using a simple stain?

A

To visualize the shape, size, and arrangement of cells.

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4
Q

Fill in the blank: A common dye used in simple staining is _____ .

A

methylene blue

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5
Q

Which of the following is NOT a common simple stain? A) Crystal violet B) Safranin C) Gram’s iodine

A

C) Gram’s iodine

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6
Q

What is the first step in the simple staining procedure?

A

Prepare a bacterial smear on a slide.

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7
Q

True or False: Simple stains require multiple reagents to produce a result.

A

False

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8
Q

What type of microscopy is typically used to view simple stains?

A

Light microscopy

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9
Q

What is the effect of heat-fixing a bacterial smear?

A

It kills the bacteria and adheres them to the slide.

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10
Q

Which of the following stains is often used for simple staining? A) Acid-fast stain B) Negative stain C) Methylene blue

A

C) Methylene blue

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11
Q

What color do cells typically appear when stained with a simple stain?

A

The color of the dye used, e.g., blue with methylene blue.

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12
Q

Fill in the blank: Simple staining is particularly useful for observing _____ of bacteria.

A

morphology

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13
Q

What is the main limitation of a simple stain?

A

It does not provide information about the type of bacteria.

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14
Q

True or False: Simple stains can be used to determine the presence of bacterial capsules.

A

False

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15
Q

What is the second step in the simple staining process after heat-fixing?

A

Apply the stain to the slide.

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16
Q

Which cellular structure is primarily visualized using a simple stain?

A

The cell wall and cell membrane.

17
Q

What is the role of the rinse step in the simple staining process?

A

To remove excess dye from the slide.

18
Q

Multiple choice: What is commonly used as a counterstain in a simple staining procedure? A) Ethanol B) Safranin C) Acetic acid

A

B) Safranin

19
Q

What is the final step after staining and rinsing the slide?

A

Observe the slide under a microscope.

20
Q

True or False: Simple stains can help identify the metabolic activity of bacteria.

21
Q

Fill in the blank: The simple stain technique is often the first step in _____ techniques.

A

bacterial identification

22
Q

What type of cells can be observed using simple staining?

A

Both prokaryotic and eukaryotic cells.

23
Q

What is the typical duration for applying a simple stain to a slide?

A

About 1-5 minutes, depending on the dye.

24
Q

What is the importance of observing the arrangement of cells in a simple stain?

A

It can indicate the type of bacteria and their growth patterns.

25
Q

True or False: Simple staining can be used to visualize intracellular structures.