Simple Stain Flashcards
What is a simple stain?
A simple stain is a basic staining technique used to highlight the morphology and arrangement of bacteria.
True or False: Simple stains can differentiate between different types of bacteria.
False
What is the primary purpose of using a simple stain?
To visualize the shape, size, and arrangement of cells.
Fill in the blank: A common dye used in simple staining is _____ .
methylene blue
Which of the following is NOT a common simple stain? A) Crystal violet B) Safranin C) Gram’s iodine
C) Gram’s iodine
What is the first step in the simple staining procedure?
Prepare a bacterial smear on a slide.
True or False: Simple stains require multiple reagents to produce a result.
False
What type of microscopy is typically used to view simple stains?
Light microscopy
What is the effect of heat-fixing a bacterial smear?
It kills the bacteria and adheres them to the slide.
Which of the following stains is often used for simple staining? A) Acid-fast stain B) Negative stain C) Methylene blue
C) Methylene blue
What color do cells typically appear when stained with a simple stain?
The color of the dye used, e.g., blue with methylene blue.
Fill in the blank: Simple staining is particularly useful for observing _____ of bacteria.
morphology
What is the main limitation of a simple stain?
It does not provide information about the type of bacteria.
True or False: Simple stains can be used to determine the presence of bacterial capsules.
False
What is the second step in the simple staining process after heat-fixing?
Apply the stain to the slide.
Which cellular structure is primarily visualized using a simple stain?
The cell wall and cell membrane.
What is the role of the rinse step in the simple staining process?
To remove excess dye from the slide.
Multiple choice: What is commonly used as a counterstain in a simple staining procedure? A) Ethanol B) Safranin C) Acetic acid
B) Safranin
What is the final step after staining and rinsing the slide?
Observe the slide under a microscope.
True or False: Simple stains can help identify the metabolic activity of bacteria.
False
Fill in the blank: The simple stain technique is often the first step in _____ techniques.
bacterial identification
What type of cells can be observed using simple staining?
Both prokaryotic and eukaryotic cells.
What is the typical duration for applying a simple stain to a slide?
About 1-5 minutes, depending on the dye.
What is the importance of observing the arrangement of cells in a simple stain?
It can indicate the type of bacteria and their growth patterns.
