Lab Flashcards
What should you do before and after using work areas in the lab?
Disinfect work areas before and after use.
What are the key safety practices in the lab?
Treat all microorganisms as potential pathogens, sterilize equipment and materials, wash hands, do not eat or drink in the lab, label everything clearly, and autoclave or disinfect all waste material.
What information should be included when labeling samples?
Date, name of microorganism, number table, afternoon class, and initials.
What is the purpose of a graduated cylinder?
A graduated cylinder is used to measure the volume of a liquid.
What is the difference between a scale and a graduated cylinder?
A scale is used for accuracy, while a graduated cylinder measures liquid volume.
What are the grading divisions for 100mL and 10mL graduated cylinders?
100mL cylinders have 1mL grading divisions, while 10mL cylinders have 0.1mL grading divisions.
What is the recommended temperature for a fridge?
A fridge should be set to maintain a temperature of 40 °F or below.
What is the temperature range for a freezer?
A freezer temperature ranges from -20 to -40 °C.
What are positive and negative controls in experiments?
Positive controls verify that an organism grows successfully, while negative controls verify the testing environment and handling.
What is the magnification capability of compound microscopes?
Compound microscopes can magnify an object by 400 times or more, up to 1,000 times.
What is the typical magnification for dissecting microscopes?
Dissecting microscopes usually magnify an object by 40 times or less.
What is immersion oil used for in microscopy?
Immersion oil is used to increase the resolving power of a microscope by immersing both the objective lens and the specimen in oil.
What is the purpose of a 4-way streak?
The purpose of a 4-way streak is to obtain single, isolated colonies of a microorganism.
What is the protocol for performing a 4-way streak?
A sterile inoculating loop is dipped into a mixed culture and streaked over the surface of the nutrient medium to separate bacteria.
How can you reduce contamination while working aseptically?
Open the lid of the agar plate just enough to streak it and minimize exposure to the environment.
What is the function of an autoclave?
The autoclave is used to sterilize materials.
What are the main types of bacterial cell morphology?
Cocci, bacilli, spirochetes, and spirillum.
What are the arrangements of bacterial cells?
Chains, tetrads, and clusters.
What terminology should be used to describe bacterial colony morphology?
Use the terms provided in the lab manual.
What are the main types of bacterial cell morphology?
Cocci, bacilli, spirochetes, and spirillum.
What are the common arrangements of bacterial cells?
Chains, tetrads, and clusters.
What terminology is used to describe bacterial colony morphology?
Terms from the lab manual.
Which genera of bacteria should be recognized for their cell morphology?
Staphylococcus, Streptococcus, Bacillus, Escherichia, Enterobacter, Pseudomonas, Salmonella, Shigella.
What are the steps involved in the Gram stain?
Understand the steps and be able to troubleshoot the Gram stain.