Steak And Pour Plate Flashcards
What is a pour plate method?
A microbiological technique used to isolate and enumerate microorganisms by mixing a sample with molten agar and pouring it into a petri dish.
True or False: The pour plate method can be used for both aerobic and anaerobic organisms.
True
What type of medium is typically used in the pour plate method?
Molten agar
Fill in the blank: The pour plate technique involves diluting a sample in a series of _____ before plating.
dilutions
What is the main advantage of using the pour plate method over the spread plate method?
It allows for the growth of anaerobic bacteria.
What is a common application of the pour plate method?
Counting viable bacteria in a sample.
Multiple Choice: Which of the following is not a step in the pour plate method? A) Dilution of the sample B) Mixing with agar C) Incubating at 100°C D) Pouring into a petri dish
C) Incubating at 100°C
What is the typical incubation temperature for the pour plate method?
37°C
True or False: The pour plate method can result in colonies growing within the agar.
True
What is the primary purpose of performing serial dilutions in the pour plate method?
To obtain a countable number of colonies.
Fill in the blank: In the pour plate method, after mixing the sample with agar, it is allowed to _____ before incubation.
solidify
What type of organisms are typically favored in the pour plate method?
Both aerobic and anaerobic organisms.
Multiple Choice: Which of the following is a disadvantage of the pour plate method? A) Time-consuming B) Cannot grow anaerobes C) Requires expensive equipment D) Difficult to isolate colonies
D) Difficult to isolate colonies
What is the main disadvantage of the pour plate method?
Colony isolation can be difficult due to overlapping colonies.
True or False: The pour plate method is suitable for all types of samples.
False
What is the typical volume of sample used in a pour plate?
1 ml to 1.0 ml
Fill in the blank: The pour plate method is often used in _____ analysis of food and water samples.
microbiological
What type of colony morphology can be observed in the pour plate method?
Various, including size, shape, and color.
Multiple Choice: Which of the following is a key factor in determining colony count accuracy in the pour plate method? A) Agar temperature B) Incubation time C) Sample dilution D) All of the above
D) All of the above
What is the role of agar in the pour plate method?
To provide a solid surface for microorganisms to grow.
True or False: The pour plate method is less commonly used than the spread plate method.
True
What is a common mistake when performing the pour plate method?
Using agar that is too hot, which can kill the microorganisms.
Fill in the blank: The pour plate method is particularly useful for determining the _____ of microorganisms in a sample.
viable count
What is the difference between the pour plate and spread plate methods?
Pour plate involves mixing the sample with agar, while spread plate involves spreading a diluted sample on the surface of agar.
Multiple Choice: Which of the following is an advantage of the pour plate method? A) Simplicity B) Cost-effectiveness C) Ability to culture anaerobes D) All of the above
D) All of the above
What is the purpose of incubating the pour plates?
To allow the microorganisms to grow and form colonies.
