Biotechology Flashcards
What is Reverse Transcriptase?
An enzyme that synthesizes cDNA from an mRNA template.
What is cDNA?
Complementary DNA made from mRNA, free of introns.
What is Hybridization?
The process of DNA or RNA pairing with complementary sequences.
What is FISH (Fluorescence In Situ Hybridization)?
A technique using fluorescent probes to detect specific genes in chromosomes.
What is PCR (Polymerase Chain Reaction)?
A method to rapidly replicate DNA using primers and DNA polymerase.
What are the steps of PCR?
Includes denaturation, priming, and extension to amplify DNA.
What is Recombinant DNA Technology?
A method to transfer genetic material from one organism to another.
What are Transgenic Organisms?
Organisms that have foreign DNA inserted into their genome.
What is Bioremediation?
Using microorganisms to remove pollutants from the environment.
What is Synthetic Biology?
Designing and constructing new biological systems.
What is Gene Therapy?
Introducing, removing, or altering genes to treat diseases.
What are Sequence Maps?
Charts that show the position of genes on a chromosome.
What is Whole Genome Shotgun Sequencing?
A sequencing method that fragments DNA and assembles it computationally.
What is High-Throughput Sequencing?
Rapid sequencing of large amounts of DNA simultaneously.
What is Genomics?
The study of entire genomes, including gene mapping and sequencing.
What is Bioinformatics?
The application of computational tools to analyze genetic data.
What does Annotating the Genome involve?
Identifying functional elements within a genome.
What is DNA Profiling?
Analyzing genetic markers to identify individuals.
What is a Single Nucleotide Polymorphism (SNP)?
A variation in a single DNA nucleotide among individuals.
What is a Microarray Assay?
A technique that measures gene expression across many genes at once.
What is Proteomics?
The study of the entire set of proteins in a cell or organism.
What is CRISPR-Cas9?
A gene-editing technology that allows for precise modifications of DNA sequences.
What is biotechnology?
Biotechnology is the use of living organisms or biological systems to develop products and technologies.
True or False: Biotechnology only involves genetic engineering.
False