We - Protein biomarker discovery and validation in Alzheimer

Question 1

What is the hook effect for assays?

Answer 1

When there’s so much protein it sticks to both the capture and the detection antibody, meaning they cannot make a good complex. Then the assay gets lower values even though the concentration is higher. This is called the hook effect

Question 2

What is the matrix effect in biomarker testing?

Answer 2

The method should be able to differentiate between the biomarker of interest and endogenous, unrelated components in the sample matrix (the sample matrix being for example, blood).

Question 3

What is parallelism in biomarker testing?

Answer 3

Investigates if the binding characteristic of the endogenous biomarker to the antibodies is the same as for the standard.

Question 4

What does it mean if the line is parallel or not parallel in biomarker testing, parallelism?

Answer 4

If the line is parallel, it has the same binding characteristics. If not, it has different binding characteristics.

Question 5

pTau is a biomarker specific for…?

Answer 5

Alzheimer’s disease.