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Hubble Notes > Virology > Flashcards

Virology Flashcards

1
Q

Short term storage temperature for viruses (3 days)

A

4C

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2
Q

Long-term storage temperature for viruses (4 days or longer)

A

-70C

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3
Q

Mature virus containing NUCLEIC ACID CORE with protein coat, with or without envelope

A

Virion

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4
Q

Viruses are charcterized by the presence of

A

DNA or RNA

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5
Q

Largest DNA virus

A

Poxvirus

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6
Q

Largest RNA virus

A

Paramyxovirus

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7
Q

Smallest DNA virus

A

Parvovirus

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8
Q

Smallest RNA virus

A

Picornaviridae (Enterovirus)

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9
Q

All DNA viruses are icosahedral except

A

Poxvirus

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10
Q

First step in the replication cycle of a virus

A

Attachment & replication

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11
Q

Where is the site of virion assembly

A

Nucleus or cytoplasm

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12
Q

Calcium alginate is TOXIC to N. gonorrhoeae and viruses because it could inactivate _____

A

HSV (Herpes Simplex Virus)

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13
Q

The swab used for collecting samples for detecting viruses should be made from

A

Dacron or rayon

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14
Q

Latency site of Varicella Zoster Virus

A

Dorsal root of ganglia

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15
Q

Tzanck smear is used to detect

A

HSV and VZV

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16
Q

Patient has chronic fatigue syndrome but negative for Streptococcus, EBV, and hepatitis. Test for

A

CMV (Cytomegalovirus)

17
Q

CMV isolation is best accomplished using

A

Human embryonic fibroblasts

18
Q

Cross-reactive in all human adenoviruses

19
Q

Eosinophilic cytoplasmic inclusions are elementary bodies of

20
Q

Reverse transcriptase is found in what type of virus

A

Retrovirus

21
Q

Specimen (s) collected from a patient with suspected enteroviral meningitis

A

Stool
Throat swab
CSF
Serum

22
Q

Enteroviruses can be differentiated from rhinoviruses by

A

Acid resistance

23
Q

Gold standard for rabies diagnosis

A

Direct fluorescent antibody test

24
Q

Myxoviridae virus envelope contains

A

Hemagglutinin & neuraminidase

25
Q

Test for influenza virus

A

Hemagglutination-inhibition

26
Q

Enteric Cytopathic Human Orphan virus is also known as

27
Q

Specimen of choice for detecting rotavirus

28
Q

What is used to collect a liquid stool sample in infants

A

Disposable diaper

29
Q

Stool sample is submitted for RSV detection. What should you do?

A

Reject the sample.
RSV = Respiratory Synctial Virus

30
Q

Virus associated with bleeding disorder following bite from an Aedes mosquito

A

Dengue virus

31
Q

Why is 2nd dengue infection more dangerous?

A

Serotype is different than 1st infection

32
Q

Specimen of choice for COVID-19 testing?

A

Nasopharyngeal swab

33
Q

TRUE OR FALSE: For COVID-19 specimen collection, synthetic fiber swabs with thin plastic or wire shafts for sampling the nasopharyngeal mucosa is used

34
Q

Method of choice for COVID-19 testing

A

Nucleic Acid Amplification (RT-PCR)

35
Q

Function of Thermocycler

A

Amplifies DNA and RNA

Hubble Notes (13 decks)

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