Virology Flashcards
Short term storage temperature for viruses (3 days)
4C
Long-term storage temperature for viruses (4 days or longer)
-70C
Mature virus containing NUCLEIC ACID CORE with protein coat, with or without envelope
Virion
Viruses are charcterized by the presence of
DNA or RNA
Largest DNA virus
Poxvirus
Largest RNA virus
Paramyxovirus
Smallest DNA virus
Parvovirus
Smallest RNA virus
Picornaviridae (Enterovirus)
All DNA viruses are icosahedral except
Poxvirus
First step in the replication cycle of a virus
Attachment & replication
Where is the site of virion assembly
Nucleus or cytoplasm
Calcium alginate is TOXIC to N. gonorrhoeae and viruses because it could inactivate _____
HSV (Herpes Simplex Virus)
The swab used for collecting samples for detecting viruses should be made from
Dacron or rayon
Latency site of Varicella Zoster Virus
Dorsal root of ganglia
Tzanck smear is used to detect
HSV and VZV
Patient has chronic fatigue syndrome but negative for Streptococcus, EBV, and hepatitis. Test for
CMV (Cytomegalovirus)
CMV isolation is best accomplished using
Human embryonic fibroblasts
Cross-reactive in all human adenoviruses
Hexon
Eosinophilic cytoplasmic inclusions are elementary bodies of
Poxvirus
Reverse transcriptase is found in what type of virus
Retrovirus
Specimen (s) collected from a patient with suspected enteroviral meningitis
Stool
Throat swab
CSF
Serum
Enteroviruses can be differentiated from rhinoviruses by
Acid resistance
Gold standard for rabies diagnosis
Direct fluorescent antibody test
Myxoviridae virus envelope contains
Hemagglutinin & neuraminidase
Test for influenza virus
Hemagglutination-inhibition
Enteric Cytopathic Human Orphan virus is also known as
Echovirus
Specimen of choice for detecting rotavirus
Stool
What is used to collect a liquid stool sample in infants
Disposable diaper
Stool sample is submitted for RSV detection. What should you do?
Reject the sample.
RSV = Respiratory Synctial Virus
Virus associated with bleeding disorder following bite from an Aedes mosquito
Dengue virus
Why is 2nd dengue infection more dangerous?
Serotype is different than 1st infection
Specimen of choice for COVID-19 testing?
Nasopharyngeal swab
TRUE OR FALSE: For COVID-19 specimen collection, synthetic fiber swabs with thin plastic or wire shafts for sampling the nasopharyngeal mucosa is used
True
Method of choice for COVID-19 testing
Nucleic Acid Amplification (RT-PCR)
Function of Thermocycler
Amplifies DNA and RNA