Mycology Flashcards
Incubation temperature and duration for fungal cultures
30C for 21 to 30 days
Muticellular phase of fungi
Mold phase
Unicellular phase of fungi
Yeast phase
Most common mold isolate
Aspergillus spp.
Most common yeast isolate
Candida spp.
When preparing temporary mounts of fungi, why is slide gently heated?
To increase rate of clearing
Most commonly used mounting fluid to prepare fungal microscopy slides
Lactophenol cotton blue
Fluorescent dye for fungi that stains the cell wall or chitin
Calcuofluor white
Fungus medium for primary isolation and maintenance of fungal culture
SDA (Sabouraud Dextrose Agar)
Antibiotics incorporated in the Mycosel medium
Chloramphenicol & cycloheximide
Stain in Aman medium
Cotton blue
Basidiospores, Ascospores, Zygospores, Oospores are examples of
Sexual spores
Spore that resulted from simple fragmentation of mycelium
Arthrospore (arthroconidia)
Coccidiodes, Aureobasidium, Trichosporon, Geotrichium are positive for _____
Arthrospore formation
Test for demonstration of spore of aerial mycelium
Cellophane tape method
“Spaghetti and meatballs” fungus
Malassezia furfur
Media containing olive oil is used to isolate
Malassezia furfur
Fungal infection of the skin, hair, and nail is called
Dermatophytosis
Dermatophytes can be demonstrated using what specimen
Skin scrapings
Skin and nails must be cleaned with _____ before the surface is scraped
70% alcohol
Positive result in hair penetration test
V-shaped hair penetration
Fungi that pose a major biohazard to laboratory personnel
Coccidioides immitis
Histoplasmosis may demonstrate positive reactions with serologic tests for
Blastomyces
Candidiasis infection affecting oral cavity
Oral thrush
Spores produced by Candida albicans
Blastospores
Chlamydospores
Other Candida spp. are commonly identified by FERMENTATION or assimilation of particular _____
Carbohydrates
Colonization by dermatophytic fungi of the hair, nails, and skin is called _____
Dermatophytoses
Diagnostic for chromoblastomycosis
Sclerotic body
Result of an antigen-antibody reaction in cases of sporothricosis?
Asteroid body
Fungi that grow on Nigerseed (Birdseed) agar
Cryptococcus neoformans
When culturing Cryptococcus on SDA, what antibiotic should not be used?
Cycloheximide
Biochemical test used to presumptively identify Cryptococcus neoformans
Urease
Stain used to demonstrate Cryptococcus neoformans
India ink
Definitive test for Cryptococcus neoformans
PCR
Fungi that are MISTAKEN AS LYMPHOCYTES in cerebrospinal fluid
Cryptococcus neoformans
Most common pathogen causing pneumonitis in AIDS patients
Pnuemocystis jirovecii (Pneumocystis carinii)
Protozoan-like fungi stained by Gomori methenamine silver stain
Pnuemocystis jirovecii (Pneumocystis carinii)