Mycology

Question 1

Incubation temperature and duration for fungal cultures

Answer 1

30C for 21 to 30 days

Question 2

Muticellular phase of fungi

Answer 2

Mold phase

Question 3

Unicellular phase of fungi

Answer 3

Yeast phase

Question 4

Most common mold isolate

Answer 4

Aspergillus spp.

Question 5

Most common yeast isolate

Answer 5

Candida spp.

Question 6

When preparing temporary mounts of fungi, why is slide gently heated?

Answer 6

To increase rate of clearing

Question 7

Most commonly used mounting fluid to prepare fungal microscopy slides

Answer 7

Lactophenol cotton blue

Question 8

Fluorescent dye for fungi that stains the cell wall or chitin

Answer 8

Calcuofluor white

Question 9

Fungus medium for primary isolation and maintenance of fungal culture

Answer 9

SDA (Sabouraud Dextrose Agar)

Question 10

Antibiotics incorporated in the Mycosel medium

Answer 10

Chloramphenicol & cycloheximide

Question 11

Stain in Aman medium

Answer 11

Cotton blue

Question 12

Basidiospores, Ascospores, Zygospores, Oospores are examples of

Answer 12

Sexual spores

Question 13

Spore that resulted from simple fragmentation of mycelium

Answer 13

Arthrospore (arthroconidia)

Question 14

Coccidiodes, Aureobasidium, Trichosporon, Geotrichium are positive for _____

Answer 14

Arthrospore formation

Question 15

Test for demonstration of spore of aerial mycelium

Answer 15

Cellophane tape method

Question 16

“Spaghetti and meatballs” fungus

Answer 16

Malassezia furfur

Question 17

Media containing olive oil is used to isolate

Answer 17

Malassezia furfur

Question 18

Fungal infection of the skin, hair, and nail is called

Answer 18

Dermatophytosis

Question 19

Dermatophytes can be demonstrated using what specimen

Answer 19

Skin scrapings

Question 20

Skin and nails must be cleaned with _____ before the surface is scraped

Answer 20

70% alcohol

Question 21

Positive result in hair penetration test

Answer 21

V-shaped hair penetration

Question 22

Fungi that pose a major biohazard to laboratory personnel

Answer 22

Coccidioides immitis

Question 23

Histoplasmosis may demonstrate positive reactions with serologic tests for

Answer 23

Blastomyces

Question 24

Candidiasis infection affecting oral cavity

Answer 24

Oral thrush

Question 25

Spores produced by Candida albicans

Answer 25

Blastospores
Chlamydospores

Question 26

Other Candida spp. are commonly identified by FERMENTATION or assimilation of particular _____

Answer 26

Carbohydrates

Question 27

Colonization by dermatophytic fungi of the hair, nails, and skin is called _____

Answer 27

Dermatophytoses

Question 28

Diagnostic for chromoblastomycosis

Answer 28

Sclerotic body

Question 29

Result of an antigen-antibody reaction in cases of sporothricosis?

Answer 29

Asteroid body

Question 30

Fungi that grow on Nigerseed (Birdseed) agar

Answer 30

Cryptococcus neoformans

Question 31

When culturing Cryptococcus on SDA, what antibiotic should not be used?

Answer 31

Cycloheximide

Question 32

Biochemical test used to presumptively identify Cryptococcus neoformans

Answer 32

Urease

Question 33

Stain used to demonstrate Cryptococcus neoformans

Answer 33

India ink

Question 34

Definitive test for Cryptococcus neoformans

Answer 34

PCR

Question 35

Fungi that are MISTAKEN AS LYMPHOCYTES in cerebrospinal fluid

Answer 35

Cryptococcus neoformans

Question 36

Most common pathogen causing pneumonitis in AIDS patients

Answer 36

Pnuemocystis jirovecii (Pneumocystis carinii)

Question 37

Protozoan-like fungi stained by Gomori methenamine silver stain

Answer 37

Pnuemocystis jirovecii (Pneumocystis carinii)