Urine Samples Flashcards

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0
Q

Explain the process of testing for anabolic steroids

A
  • a urine sample is analysed using gas chromatography or mass spectroscopy
  • in gas chromatography a substance is vaporised in the presence of a gaseous solvent
  • it is passed down a long thing tube coated with an adsorption agent
  • different substance stay dissolved in the the gas for different periods of time (retention time) and reaves different distances down the tube
  • when the substance is no longer dissolved it will adsorb to the side of the tube which contains the adsorption agent
  • this is then analysed to create a chromatogram which is compared and analysed to know values for different substances
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1
Q

Explain the process of pregnancy testing

A
  • once implanted in the uterine lining, a human embryo starts secreting a pregnancy hormone called human chorionic gonadotrophin (hCG)
  • a pregnancy test will test for hCG
  • antibodies on the pregnancy test will have a complementary shape to the hCG
  • hCG will bind to the antibody
  • the antibody has a coloured bead attached to it
  • it forms a hCG-antibody complex which moves up the strip until it stick so a band of immobilised antibodies
  • as a result all the antibodies carrying a coloured bead and attached to hCG are held in one place forming a coloured line
  • there is always one control coloured line to use for comparison and a second coloured line indicates pregnancy
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