Evaluating The Evidence For Chemiosmosis Flashcards

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0
Q

Explain why no ATP is made in mitoblasts (mitochondria stripped of outer membrane)

A
  • there is no inter membrane space so H+ ions/protons are not trapped so they do not build up
  • so there is no gradient formed and a gradient is needed to make ATP
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1
Q

Explain why there is a lower pH in the intermembrane space

A
  • inner membrane is impermeable as H+ ions are accumulating
  • there is a potential difference across the membrane and the matrix as a loss of H+ from the matrix makes it more negative, a gain of H+ in the intermembrane space makes it more positive
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2
Q

Explain why there is no ATP made in the presence of oligomycin (binds to ATP synthase, blocking the channel)

A
  • H+ ions cannot flow down the gradient through the ATP synthase channel
  • so they cannot produce a proton motive force –> this showed that the flow of H+ ions through ATP synthase was needed to make ATP
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3
Q

Explain why no ATP is made of the head pieces are removed from the stalked particles

A

• if there is no heads the cannot rotate under proton motive force to phosphorylate ADP + Pi –> heads make ATP

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