Unit One

Question 1

what was used to be a light source for the light microscope back in the day

Answer 1

flame or direct sunlight

Question 2

robert hooke discovered what under the light microscope

Answer 2

cork cells

Question 3

when did robert hook discover cork cells

Answer 3

1665

Question 4

what term did robert hooke coin

Answer 4

cell theory

Question 5

what is the cell theory

Answer 5

life comes from life

Question 6

what are the four things that can be seen under the microscope

Answer 6

1. cell sizes
2. movement, shape changes, cell division
3. cell staining
4. cellular anatomy

Question 7

what level of magnification can the naked eye see

Answer 7

2 mm

Question 8

to see cells what is the magnification range

Answer 8

.2 mm to 2 micrometers

Question 9

to see organelles what is the magnification range

Answer 9

a little over 2 micrometers to a little under 200 nm

Question 10

what magnification is needed for minimum resolvable by light microscope

Answer 10

200 nm

Question 11

to see molecules, what is the magnification range

Answer 11

over 20 nm to .2 nm

Question 12

what magnification is needed for the minimum resolvable by electron microscope

Answer 12

.2 nm

Question 13

to see atoms what is the magnification range

Answer 13

.2 nm and less

Question 14

light microscopy has magnification up to

Answer 14

1000x

Question 15

light microscopy has a resolution of

Answer 15

.2 mm

Question 16

how does light microscopy work

Answer 16

light source is put through a condenser that focuses it through a specimen and objective to be seen through eye piece

Question 17

what is resolution

Answer 17

ability of microscope to distinguish two separate objects based on how close

Question 18

what is fluorescent microscopy

Answer 18

uses fluorescent staining to see specimen and filters isolate correct wavelength

Question 19

what is confocal microscopy

Answer 19

uses laser as light source, this provides more detail

Question 20

what image does a confocal microscope create

Answer 20

2-D image, but computer can interpret to 3-D

Question 21

What is transmission electron microscopy

Answer 21

transmits beam of electrons

Question 22

what is the magnification and resolution of a transmission electron microscope

Answer 22

magnification of 1 million and resolution of 2 nm

Question 23

what is scanning electron microscopy

Answer 23

coats the specimen with a heavy metal (gold coin) that produces a 3-D image

Question 24

what is the magnification of a scanning electron microscope

Answer 24

1 million