Unit One Flashcards
what was used to be a light source for the light microscope back in the day
flame or direct sunlight
robert hooke discovered what under the light microscope
cork cells
when did robert hook discover cork cells
1665
what term did robert hooke coin
cell theory
what is the cell theory
life comes from life
what are the four things that can be seen under the microscope
- cell sizes
- movement, shape changes, cell division
- cell staining
- cellular anatomy
what level of magnification can the naked eye see
2 mm
to see cells what is the magnification range
.2 mm to 2 micrometers
to see organelles what is the magnification range
a little over 2 micrometers to a little under 200 nm
what magnification is needed for minimum resolvable by light microscope
200 nm
to see molecules, what is the magnification range
over 20 nm to .2 nm
what magnification is needed for the minimum resolvable by electron microscope
.2 nm
to see atoms what is the magnification range
.2 nm and less
light microscopy has magnification up to
1000x
light microscopy has a resolution of
.2 mm
how does light microscopy work
light source is put through a condenser that focuses it through a specimen and objective to be seen through eye piece
what is resolution
ability of microscope to distinguish two separate objects based on how close
what is fluorescent microscopy
uses fluorescent staining to see specimen and filters isolate correct wavelength
what is confocal microscopy
uses laser as light source, this provides more detail
what image does a confocal microscope create
2-D image, but computer can interpret to 3-D
What is transmission electron microscopy
transmits beam of electrons
what is the magnification and resolution of a transmission electron microscope
magnification of 1 million and resolution of 2 nm
what is scanning electron microscopy
coats the specimen with a heavy metal (gold coin) that produces a 3-D image
what is the magnification of a scanning electron microscope
1 million
