Unit 4: Concept 2 - Protein Synthesis Flashcards
What is protein synthesis?
reads instructions to make polypeptides
What are polypeptides?
chain of amino acids, folds into proteins
What is the central dogma of genetics?
proteins made is ribosomes
What are the 2 types of central dogma of genetics?
transcription and translation
What are the three types of RNA?
mRNA (messenger),
tRNA (binder),
rRNA (makes)
What is the purpose, location, start and end of transcription?
P: carry instructions out of nucleus,
L: nucleus,
S: DNA,
E: mRNA
What is the process of transcription?
- RNA polymerase binds to DNA promoter and unzips the gene that needs copying,
- RNA polymerase uses complementary base-pairing rules to match RNA nucleotides with exposed DNA nucleotides,
- Release completed mRNA,
- DNA zips back up and the mRNA leaves the nucleus and enters the cytoplasm
Match the following:
DNA: ‘3 TACGCTAGTACGATT ‘5
RNA: ‘5 AUGCGAUCAUGCUAA ‘3
What is RNA splicing?
mRNA has introns and exons (coding regions)
What is the genetic code?
instructions for proteins
What is a codon?
3 nucleotides on mRNA
What is an anti-codon?
complementary 3 nucleotides of tRNA
What is an amino acid?
monomers for making proteins
What is the purpose, location, start and end of translation?
P: read instructions to make polypeptides,
L: ribosomes,
S: mRNA,
E: polypeptide
What is the process of translation?
- mRNA attaches to the small subunit of ribosomes,
- Ribosome reads the mRNA codons in the ‘5 to ‘3 direction, starting with the “AUG” codon,
- tRNAs act like taxis to pick up and drop off the amino acids that match with each codon,
- tRNAs continue to drop off amino acids and the ribosomes bind the amino acids together with peptide bonds,
- When the “STOP” codon is reached, the ribosome releases the completed polypeptide chain