Unit 1: 6.3 Isolation, Culture, and Identication Of Viruses Flashcards
1
Q
What does a viral cultivation require the presence of
A
They require some form of a host cell
- whole organism
- embryo
- cell culture
2
Q
How can viruses be isolated?
A
Viruses can be isolated from samples by filtration
3
Q
What is viral filtrate?
A
A rich source of released virions
4
Q
How are bacteriophages detected?
A
By the presence of clear plaques on bacterial lawn
5
Q
How are animal and plant viruses detected?
A
- cytopathic effects
- molecular techniques (PCR, RT-PCR)
- enzyme immunoassays
- serological assays (hemaggluation assay)
6
Q
Why are viruses originally described as filterable agents?
A
They were smaller than bacteria and could be filtered to separate them
