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PSALM GIRLS: HISTOPATHOLOGY > TRIMMING 2.0 > Flashcards

TRIMMING 2.0 Flashcards

includes ADHESIVES 💫

1
Q

preserving tissues by rapid freezing and removing water by a physical process from the tissue block WITHOUT THE USE OF ANY CHEMICAL FIXATIVE

A

FREEZE-DRYING

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2
Q

FREEZE DRYING:

preserving tissues by rapid freezing

A

QUENCHING

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3
Q

FREEZE-DRYING:

removing water

A

DESICCATION

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4
Q

tissue thickness in FREEZE-DRYING

A

2mm

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5
Q

COMPLETE PROCESSING TIME in Freeze-Drying

A

24-48 HOURS

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6
Q

temperature in QUENCHING

A

-160°C

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7
Q

method wherein the tissue is fixed in ROSSMAN’S FLUID or in 1% ACETONE and dehydrated in absolute alcohol

A

FREEZE-SUBSTITUTION

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8
Q

COMPOSITION of ROSSMAN’S FLUID

A

SATURATED PICRIC ACID + FORMALDEHYDE

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9
Q

involves the REMOVAL OF GROSS NICKS and IRREGULARITIES on the knife edge

A

HONING

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10
Q

direction of HONING

A

HEEL TO TOE

1st letter of HONING: H; so HEEL comes first before toe

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11
Q

HEEL vs TOE

handle end

A

HEEL

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12
Q

HEEL vs TOE

HEAD PORTION; part of the knife farthest from handle

A

TOE

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13
Q

a natural sharpening stone or a hard grinding surface for sharpening a knife or other cutting tools

A

HONE

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14
Q

EXCELLENT ALTERNATIVE for hone

A

PLATE GLASS

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15
Q

used only for BADLY NICKED KNIVES; available in a wide variety of fineness

A

CARBORUNDUM HONES

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16
Q

gives more polishing effect than the Belgian Yellow; a stone of medium fineness

A

ARKANSAS STONE

17
Q

the FINEST stone; gives the best result

A

YELLOW BELGIAN or BELGIAN YELLOW

18
Q

also called OIL STONES

A

BELGIAN BLACK OIL

19
Q

under BELGIAN BLACK OIL:

oils used as lubricants

A
  1. LIGHT MACHINE OIL
  2. LIQUID PARAFFIN OIL (mineral oil)
  3. VEGETABLE OIL
  4. NATURAL OIL
  5. XYLENE

“LLVNX” lalavan x??? 😂

20
Q

the process whereby the “BURR” formed during honing is removed and the cutting edge of the knife is polished

A

STROPPING

21
Q

direction of STROPPING

A

TOE to HEEL

22
Q

the paddle strop used in HONING is made up of what material?

A

HORSE LEATHER

23
Q

knives used for trimming and SEMI-THIN SECTIONING of tissue blocks for ELECTRON MICROSCOPY

A

GLASS KNIVES

24
Q

knives used to cut any type of RESIN BLOCK for ELECTRON MICROSCOPY (brittle and expensive but very durable)

A

DIAMOND KNIVES

25
Q

a circular, thermostatically controlled bath 10-12 inches in diameter and 3-4 inches in depth

A

FLOATING-OUT BATH

26
Q

what color should the inside surface of the floating-out bath be to enable easier visualization of sections?

A

BLACK

27
Q

tiny pieces of UNRELATED TISSUE which commonly cause problems in interpretation of the biopsies by the pathologist

A

FLOATERS

28
Q

used in order to promote adhesion of sections to the slides

A

ADHESIVES

29
Q

MOST COMMONLY USED ADHESIVE because it is very easy to make, convenient, and relatively inexpensive

A

MAYER’S EGG ALBUMIN

30
Q

composition of MAYER’S EGG ALBUMIN

A
  1. EGG WHITE
  2. GLYCERIN
  3. THYMOL CRYSTALS
31
Q

what component of Mayer’s Egg Albumin prevents mold formation?

A

THYMOL CRYSTALS

32
Q

composition of DRIED ALBUMIN

A
  1. DRIED ALBUMIN
  2. SODIUM CHLORIDE
  3. DISTILLED WATER
  4. THYMOL CRYSTALS
33
Q

composition of 1% GELATIN

A
  1. GELATIN
  2. GLYCEROL
  3. DISTILLED WATER
  4. PHENOL CRYSTALS
34
Q

composition of GLUTARALDEHYDE-FORMALDEHYDE MIXTURE

A
  1. 1% GELATIN
  2. 2% FORMALDEHYDE
35
Q

composition of STARCH PASTE

A
  1. POWDERED STARCH
  2. DISTILLED WATER
  3. HYDROCHLORIC ACID
  4. THYMOL CRYSTALS
36
Q

adhesive readily available from outdated blood stored in blood banks, dispensed into sterile tubes of 0.5mL each

A

PLASMA

37
Q

used as a section adhesive in IMMUNOHISTOCHEMISTRY

A

POLY-L-LYSINE

38
Q

an adhesive which is very useful in cytology particularly for CYTOSPIN PREPARATIONS OF PROTEINACEOUS or BLOODY MATERIAL

A

3-APES

3-AMINOPROPYLTHRIETHOXYSILANE

PSALM GIRLS: HISTOPATHOLOGY (11 decks)

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