TRIMMING 2.0

Question 1

preserving tissues by rapid freezing and removing water by a physical process from the tissue block WITHOUT THE USE OF ANY CHEMICAL FIXATIVE

Answer 1

FREEZE-DRYING

Question 2

FREEZE DRYING:

preserving tissues by rapid freezing

Answer 2

QUENCHING

Question 3

FREEZE-DRYING:

removing water

Answer 3

DESICCATION

Question 4

tissue thickness in FREEZE-DRYING

Answer 4

2mm

Question 5

COMPLETE PROCESSING TIME in Freeze-Drying

Answer 5

24-48 HOURS

Question 6

temperature in QUENCHING

Answer 6

-160°C

Question 7

method wherein the tissue is fixed in ROSSMAN’S FLUID or in 1% ACETONE and dehydrated in absolute alcohol

Answer 7

FREEZE-SUBSTITUTION

Question 8

COMPOSITION of ROSSMAN’S FLUID

Answer 8

SATURATED PICRIC ACID + FORMALDEHYDE

Question 9

involves the REMOVAL OF GROSS NICKS and IRREGULARITIES on the knife edge

Answer 9

HONING

Question 10

direction of HONING

Answer 10

HEEL TO TOE

1st letter of HONING: H; so HEEL comes first before toe

Question 11

HEEL vs TOE

handle end

Answer 11

HEEL

Question 12

HEEL vs TOE

HEAD PORTION; part of the knife farthest from handle

Answer 12

TOE

Question 13

a natural sharpening stone or a hard grinding surface for sharpening a knife or other cutting tools

Answer 13

HONE

Question 14

EXCELLENT ALTERNATIVE for hone

Answer 14

PLATE GLASS

Question 15

used only for BADLY NICKED KNIVES; available in a wide variety of fineness

Answer 15

CARBORUNDUM HONES

Question 16

gives more polishing effect than the Belgian Yellow; a stone of medium fineness

Answer 16

ARKANSAS STONE

Question 17

the FINEST stone; gives the best result

Answer 17

YELLOW BELGIAN or BELGIAN YELLOW

Question 18

also called OIL STONES

Answer 18

BELGIAN BLACK OIL

Question 19

under BELGIAN BLACK OIL:

oils used as lubricants

Answer 19

1. LIGHT MACHINE OIL
2. LIQUID PARAFFIN OIL (mineral oil)
3. VEGETABLE OIL
4. NATURAL OIL
5. XYLENE

“LLVNX” lalavan x??? 😂

Question 20

the process whereby the “BURR” formed during honing is removed and the cutting edge of the knife is polished

Answer 20

STROPPING

Question 21

direction of STROPPING

Answer 21

TOE to HEEL

Question 22

the paddle strop used in HONING is made up of what material?

Answer 22

HORSE LEATHER

Question 23

knives used for trimming and SEMI-THIN SECTIONING of tissue blocks for ELECTRON MICROSCOPY

Answer 23

GLASS KNIVES

Question 24

knives used to cut any type of RESIN BLOCK for ELECTRON MICROSCOPY (brittle and expensive but very durable)

Answer 24

DIAMOND KNIVES

Question 25

a circular, thermostatically controlled bath **10-12 inches in diameter** and **3-4 inches in depth**

Answer 25

FLOATING-OUT BATH

Question 26

what color should the inside surface of the floating-out bath be to enable easier visualization of sections?

Answer 26

BLACK

Question 27

tiny pieces of **UNRELATED TISSUE** which commonly cause problems in interpretation of the biopsies by the pathologist

Answer 27

FLOATERS

Question 28

used in order to promote adhesion of sections to the slides

Answer 28

ADHESIVES

Question 29

**MOST COMMONLY USED ADHESIVE** because it is very easy to make, convenient, and relatively inexpensive

Answer 29

MAYER'S EGG ALBUMIN

Question 30

composition of **MAYER'S EGG ALBUMIN**

Answer 30

1. EGG WHITE 2. GLYCERIN 3. THYMOL CRYSTALS

Question 31

what component of Mayer's Egg Albumin prevents mold formation?

Answer 31

THYMOL CRYSTALS

Question 32

composition of **DRIED ALBUMIN**

Answer 32

1. DRIED ALBUMIN 2. **SODIUM CHLORIDE** 3. DISTILLED WATER 4. **THYMOL CRYSTALS**

Question 33

composition of **1% GELATIN**

Answer 33

1. GELATIN 2. GLYCEROL 3. DISTILLED WATER 4. **PHENOL CRYSTALS**

Question 34

composition of **GLUTARALDEHYDE-FORMALDEHYDE MIXTURE**

Answer 34

1. 1% GELATIN 2. 2% FORMALDEHYDE

Question 35

composition of **STARCH PASTE**

Answer 35

1. POWDERED STARCH 2. DISTILLED WATER 3. HYDROCHLORIC ACID 4. THYMOL CRYSTALS

Question 36

adhesive readily available from outdated blood stored in blood banks, dispensed into sterile tubes of 0.5mL each

Answer 36

PLASMA

Question 37

used as a section adhesive in **IMMUNOHISTOCHEMISTRY**

Answer 37

POLY-L-LYSINE

Question 38

an adhesive which is very useful in cytology particularly for **CYTOSPIN PREPARATIONS OF PROTEINACEOUS or BLOODY MATERIAL**

Answer 38

3-APES | 3-AMINOPROPYLTHRIETHOXYSILANE