Brainscape's Knowledge GenomeTM


Top Flashcards (Certified)
All Flashcards

PSALM GIRLS: HISTOPATHOLOGY > FIXATIVES 2.0 > Flashcards

FIXATIVES 2.0 Flashcards

PICRIC ACID FIXATIVES, GLACIAL ACETIC ACID, ALCOHOLIC FIXATIVES, OSMIUM TETROXIDE, TRICHLOROACETIC ACID, ACETONE, MICHEL'S SOLUTION, HEAT FIXATION, extras

1
Q

Good fixative for CONNECTIVE TISSUES and GLYCOGEN

A

PICRIC ACID FIXATIVES

CLUE: GLYCOGEN!!!

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

recommended for immunostaining of BIOGENIC and POLYPEPTIDE HORMONES

A

PICRIC ACID FIXATIVES

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Picric Acid fixatives dye the tissues what color?

A

YELLOW

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

class of fixatives suitable for ANILINE STAINS & TRICHROME METHOD

A

PICRIC ACID FIXATIVES

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

the chemical name for GENERAL PICRIC ACID FIXATIVES

A

2,4,6-TRINITROPHENOL

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Picric Acid is HIGHLY EXPLOSIVE when _______

A

DRY

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Picric Acid must be kept moist with __________ or __________

A

DISTILLED WATER; SATURATED ALCOHOL

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Picrates are soluble in what substance?

A

WATER

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Picrates should be transferred directly to what substance?

A

70% ALCOHOL

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

PICRIC ACID FIXATIVES: “BBHoG”

A
  1. BOUIN’S SOLUTION
  2. BRASIL’S ALCOHOLIC PICROFORMOL FIXATIVE
  3. HOLLANDE’S SOLUTION
  4. GENDRE’S SOLUTION
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

fixative for EMBRYOS and PITUITARY BIOPSIES

A

BOUIN’S SOLUTION

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

recommended for GIT BIOPSIES, ANIMAL EMBRYOS, and ENDOCRINE GLAND TISSUE

A

BOUIN’S SOLUTION

BIOPSIES, B una! vs SPX na S… and Bouin’s comes first before Hollande’s…

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

EXCELLENT fixative for GLYCOGEN demonstration

A

BOUIN’S SOLUTION

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

PREFERRED fixative for MASSON’S TRICHROME staining for collagen, elastic fibers, or connective tissues

A

BOUIN’S SOLUTION

“si Masson ug si Bouin…“ 😤

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

this fixative is NOT suitable for fixing kidney structures, lipid, and mucus

A

BOUIN’S SOLUTION

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

BEST ROUTINE fixative for GLYCOGEN

A

BRASIL’S ALCOHOLIC PICROFORMOL FIXATIVE

“BR: Best Routine; BR: BRasil”

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

overnight tissue fixation by automatic processing technique may utilize how many changes of Brasil’s fixative?

A

3-4 changes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

recommended for GIT SPECIMENS and fixation of ENDOCRINE TISSUES

A

HOLLANDE’S SOLUTION

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

RECOMMENDED for the preservation of GLYCOGEN and other carbohydrates

A

GENDRE’S SOLUTION

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Picric Acid fixative that DOES NOT REQUIRE WASHING OUT

A

GENDRE’S SOLUTION

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

in using Gendre’s Solution…

after fixation in Gendre’s Solution, the tissue is placed into what?

A

70% ETHANOL

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

Glacial Acetic Acid FREEZES and SOLIDIFIES at what temperature?

A

17°C

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

fixes and precipitates NUCLEOPROTEINS

A

GLACIAL ACETIC ACID

medyo sosyal lang paminawon ang GLACIAL ug NUCLEOPROTEINS 🤳

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

It is contraindicated for cytoplasmic fixation because it destroys MITOCHONDRIA and GOLGI ELEMENTS

A

GLACIAL ACETIC ACID

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
fixatives that denature and precipitate protein by destroying hydrogen and other bonds
ALCOHOLIC FIXATIVES
26
what concentration of alcohol is used **TO FIX AND PRESERVE GLYCOGEN, PIGMENTS, BLOOD, TISSUE FILMS, and SMEARS**?
100%
27
what concentration of alcohol and mixture are used to **PRESERVE THE COLOR OF THE SPECIMEN FOR PHOTOGRAPHIC WORK**?
80% ; GLYCERIN-ALCOHOL MIXTURE
28
may be used both as a **FIXATIVE and DEHYDRATING AGENT**
ALCOHOLIC FIXATIVES
29
* MAJOR DISADVANTAGE OF ALCOHOLIC FIXATIVES * glycogen granules move towards the poles or ends of the cells
POLARIZATION
30
used as a diluent in Wright's stain
100% METHANOL
31
**EXCELLENT** for fixing thin layer preparations such as **WET & DRY SMEARS, bone marrow tissues, and cell cultures**
100% METHANOL
32
it is used for fixing **TOUCH PREPARATIONS**
95% ISOPROPYL ALCOHOL
33
alcoholic fixative used in **70-100% CONCENTRATION**
ETHYL ALCOHOL
34
lower concentrations (70-80%) of this fixative will cause **RBC lysis & inadequate WBC preservation**
ETHYL ALCOHOL
35
used for **HISTOCHEMISTRY** especially for **ENZYME STUDIES** since it preserves **NUCLEOPROTEINS & NUCLEIC ACIDS**
ETHYL ALCOHOL
36
preserves but **DOES NOT FIX GLYCOGEN**
ETHYL ALCOHOL
37
for histochemical detection of **SODIUM URATE CRYSTALS** in patients with gout
ETHYL ALCOHOL
38
fixative that gives the **MOST USABLE DNA FRAGMENTS FOR PCR**
ETHYL ALCOHOL
39
can be both used as a **SIMPLE and COMPOUND FIXATIVE**
ETHYL ALCOHOL
40
the **MOST RAPID TISSUE FIXATIVE**
CARNOY'S FLUID
41
recommended for fixing **CHROMOSOMES, LYMPH GLANDS, and *URGENT BIOPSIES***
CARNOY'S FLUID
42
alcoholic fixative used to fix **BRAIN TISSUE for RABIES DIAGNOSIS**
CARNOY'S FLUID
43
alcoholic fixative that preserves **NISSL** and cytoplasmic granules and very suitable for small tissue fragments such as curettings and biopsy materials
CARNOY'S FLUID
44
it can be used for fixation or post-fixation of **LARGE FATTY SPECIMENS**
ALCOHOLIC FORMALIN
45
used to complete fixation after incomplete primary formalin fixation
ALCOHOLIC FORMALIN
46
for fixing ***MUCOPOLYSACCHARIDES*** and nuclear proteins
NEWCOMER'S FLUID "**Newcomer** siya kay siya lang nagmention diri ug ***MUCOPOLYSACCHARIDES***" BRAH IDK ANYMORE!!! 😴
47
it is both a **NUCLEUS and HISTOCHEMICAL FIXATIVE**
NEWCOMER'S FLUID
48
alcoholic fixative used on **FROZEN SECTION** AND SMEAR
CLARKE'S SOLUTION "ang **FROZEN** **SMEAR** ni Clarke" teh ambot lang 🥴
49
it **PRESERVES NUCLEIC ACIDS *but extracts lipids***
CLARKE'S SOLUTION **"naga *EXTRACT*** **si Clarke"** 🤑
50
FORMULA FOR ***CLARKE'S SOLUTION***
ABSOLUTE ALCOHOL & GLACIAL ACETIC ACID
51
a faster acting agent than alcoholic formalin due to the presence of acetic acid that can also produce formalin pigment
FORMOL-ACETIC ACID ALCOHOL
52
sometimes used to fix **DIAGNOSTIC CRYOSTAT SECTIONS**
FORMOL-ACETIC ACID ALCOHOL
53
alcoholic fixative: faster fixation and is used in **FROZEN SECTIONS FOR RAPID DIAGNOSIS**
GENDRE'S FIXATIVE
54
used to fix **SPUTUM**
GENDRE'S FIXATIVE
55
good preservation of **GLYCOGEN & MICRO-INCINERATION TECHNIQUE**
GENDRE'S FIXATIVE
56
a **PALE YELLOW POWDER** which dissolves in water to form a strong oxidizing solution which causes the complete denaturation of protein
OSMIUM TETROXIDE
57
What concentration of osmium tetroxide is buffered at pH 7.3-7.5?
1% OsO4
58
a good fixative and **EXCELLENT STAIN FOR LIPIDS** in MEMBRANOUS STRUCTURES AND VESICLES
OSMIUM TETROXIDE
59
prolonged exposure to this fixative causes ***CONJUNCTIVITIS and BLINDNESS***
OSMIUM TETROXIDE (or OSMIC ACID)
60
the **MOST COMMONLY USED CHROME-OSMIUM ACETIC ACID**
FLEMMING'S SOLUTION
61
# under OsO4: excellent fixative for **NUCLEAR STRUCTURES such as CHROMOSOMES**
FLEMMING'S SOLUTION
62
# under OsO4: **PERMANENTLY** fixes **FATS/LIPIDS**
FLEMMING'S SOLUTION
63
# under OsO4: recommended for **CYTOPLASMIC STRUCTURES such as MITOCHONDRIA**
FLEMMING'S SOLUTION ***WITHOUT ACETIC ACID***
64
can be used as a **WEAK DECALCIFYING AGENT**
TRICHLOROACETIC ACID
65
used for **PRECIPITATION of *PROTEINS and NUCLEIC ACIDS***
TRICHLOROACETIC ACID ## Footnote 3 words ang **PROTEINS, NUCLEIC, ACIDS** so **TRI...**; GIKAPOY NAKO ANI NA CARD DI NAKO MAALALA
66
causes marked **SWELLING EFFECT** on tissues (counteract SHRINKAGE produced by other fixatives)
TRICHLOROACETIC ACID
67
used as **FIXATIVE and DECALCIFIER** in microscopy
TRICHLOROACETIC ACID
68
at what temperature range is acetone used?
-5°C to 4°C (or 0 to 4°C)
69
recommended for the study of ***WATER DIFFUSIBLE ENZYMES*** especially **LIPASES & PHOSPHATASES**
ACETONE
70
used in fixing **BRAIN TISSUES for dx of RABIES (Negri Bodies)** | aside from Carnoy's fluid!
ACETONE
71
used in **FREEZE-SUBSTITUTION** techniques as a solvent for certain metallic salts
ACETONE
72
**NOT RECOMMENDED** as morphological fixative for tissue blocks
ACETONE
73
provide a **STABLE MEDIUM TRANSPORT OF FRESH, UNFIXED TISSUES**
MICHEL'S SOLUTION
74
this procedure involves thermal coagulation of tissue proteins for rapid diagnosis
HEAT FIXATION
75
this method is usually employed for **FROZEN TISSUE SECTIONS** and preparations of **BACTERIOLOGIC SMEARS**
HEAT FIXATION
76
# MIXTURE OF FIXATIVES FOR EM: **PARAFORMALDEHYDE-GLUTARALDEHYDE SOLUTION**
KARNOVSKY'S
77
# MIXTURE OF FIXATIVES FOR EM: mixture with **GLUTARALDEHYDE OR FORMALDEHYDE**
ACROLEIN
78
2 other terms for **POST-CHROMATIZATION**
POST-CHROMING / POST-MORDANTING
79
a secondary fixation whereby a primarily fixed tissue is placed in potassium dichromate for 24hrs, to act as mordant and aid in cytological preservation of tses
POST-CHROMATIZATION
80
the process of **REMOVING EXCESS FIXATIVE** from the tissue after fixation in order to improve staining and remove artefacts from the tses
WASHING OUT
81
**TAP WATER** is used to remove what substances?
"COF" (**CHROMATES, OSMIC ACID, FORMALIN**) ***"tap water ang gamiton para matanggal imong Circle Of Friends" 😭***
82
used to remove excess amount of **PICRIC ACID**
50-70% ALCOHOL
83
used to remove excessive **MERCURY**
ALCOHOLIC IODINE

PSALM GIRLS: HISTOPATHOLOGY (11 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions