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PSALM GIRLS: HISTOPATHOLOGY > FIXATIVES 1.0 > Flashcards

FIXATIVES 1.0 Flashcards

ALDEHYDE, METALLIC, CHROMATE, LEAD

1
Q

Formaldehyde is a gas produced by the oxidation of what substance?

A

METHYL ALCOHOL

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2
Q

REAGENT-GRADE FORMALDEHYDE contains __________

A

10% METHANOL

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3
Q

REMOVAL OF FORMALIN PIGMENTS:

this method involves placing formaldehyde fixed specimens in ACETONE, 28% AMMONIA WATER, and HYDROGEN PEROXIDE

A

LILLIE’S METHOD
(arte si Lillie so daghan siya’g kailangan 🤩)

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4
Q

REMOVAL OF FORMALIN PIGMENTS:

this is a method of formaldehyde clearance involving 70% ETHANOL and 28% AMMONIA WATER

A

KARDASEWITCH’S METHOD

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5
Q

rinsing agent in LILLIE’S METHOD

A

70% ALCOHOL

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6
Q

TRADITIONALLY, it is the most commonly used fixative in PATHOLOGY

A

10% FORMOL-SALINE

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7
Q

BEST FIXATIVE FOR CENTRAL NERVOUS TISSUES and GENERAL POST-MORTEM TISSUES for histochemical examination

A

10% FORMOL-SALINE

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8
Q

10% Formol-Saline should be changed every how many months so large specimens can be fixed for a long time?

A

3 MONTHS

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9
Q

Ideal for SILVER IMPREGNATION and other staining techniques

A

10% FORMOL-SALINE

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10
Q

It is used to preserve PHOSPHOLIPIDS

A

CALCIUM ACETATE FORMALIN (LILLIE’S)

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11
Q

REPLACED FORMOL-SALINE as the most commonly used fixative in pathology

A

CALCIUM ACETATE FORMALIN (LILLIE’S)

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12
Q

MOST WIDELY USED FIXATIVE FOR ROUTINE HISTOLOGY

A

10% NBF

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13
Q

BEST GENERAL TISSUE FIXATIVE

A

10% NBF

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14
Q

BEST FIXATIVE FOR FROZEN SECTIONS

A

10% NBF

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15
Q

the fixative of choice for many other procedures that require paraffin embedding, including IMMUNOHISTOCHEMISTRY and interphase FISH

A

10% NBF

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16
Q

BEST FIXATIVE FOR IRON-CONTAINING PIGMENTS and ELASTIC FIBERS

A

10% NBF

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17
Q

recommended for ROUTINE POST-MORTEM TISSUES

A

FORMOL CORROSIVE

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18
Q

EXCELLENT for SILVER RETICULUM METHODS

A

FORMOL CORROSIVE

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19
Q

in using formol corrosive, tissue sections should NOT BE MORE THAN how many cm thick?

A

1cm

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20
Q

a fixative that contains ALCOHOL SATURATED WITH PICRIC ACID

A

ALCOHOLIC FORMALIN

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21
Q

ALCOHOLIC FORMALIN is also known as?

A

GENDRE’S FIXATIVE

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22
Q

it fixes and dehydrates at the same time and fixes SPUTUM since it coagulates mucus

A

ALCOHOLIC FORMALIN

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23
Q

gives improved results with immunohistochemistry

A

UNBUFFERED ZINC FORMALIN

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24
Q

suitable for paraffin embedding and sectioning, and also for immunohistochemical analysis

A

PARAFORMALDEHYDE

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25
suitable for use when preparing samples for light microscopy in RESIN embedding and sectioning, and for EM
KARNOVSKY'S FIXATIVE ***"nag RES(t) siya sa KAR ni NOVa"***
26
GOOD FIXATIVE FOR NERVOUS TISSUE (ASTROCYTES)
CAJAL'S FORMOL-AMMONIUM BROMIDE
27
used for the PRESERVATION OF LIPIDS SINCE MOST FORMALIN FIXATIVES ARE INERT TO LIPIDS
BAKER'S FORMOL CALCIUM
28
it is made up of 2 FORMALDEHYDE RESIDUES, LINKED BY A THREE-CARBON CHAIN
GLUTARALDEHYDE
29
concentration of glutaraldehyde for **SMALL TISSUE FRAGMENTS and NEEDLE BIOPSIES** for 2-4 hours at room temperature
2.5%
30
concentration of glutaraldehyde for **LARGE TISSUE FRAGMENTS LESS THAN 4mm THICK** for 6-8 hours up to 24 hours
4%
31
fixative **RECOMMENDED FOR HISTOCHEMISTRY** and **ELECTRON MICROSCOPY**
GLUTARALDEHYDE
32
The MOST EFFECTIVE ALDEHYDE for PROTEIN CROSS-LINKING
GLUTARALDEHYDE
33
the MOST COMMON METALLIC FIXATIVE and WIDELY USED AS A SECONDARY FIXATIVE
MERCURIC CHLORIDE
34
Mercuric Chloride is frequently used in saturated aqueous solution of __________
5-7%
35
the routine fixative of choice for preservation of cell detail in TISSUE PHOTOGRAPHY
MERCURIC CHLORIDE
36
group of fixatives recommended for RENAL TSES, FIBRIN, CONNECTIVE TSES, and MUSCLES
MERCURIC CHLORIDE
37
fixative where its best application is for fixation of **HEMATOPOIETIC and RES TISSUES**
MERCURIC CHLORIDE
38
mercuric chloride fixative: **MERCURIC CHLORIDE + GLACIAL ACETIC ACID** just ***BEFORE*** its use
ZENKER'S FLUID
39
may act as a MORDANT to make certain special staining possible
ZENKER'S FLUID
40
recommended for PTAH and TRICHROME STAINING
ZENKER'S FLUID
41
mercuric chloride fixative: **MERCURIC CHLORIDE + 40% FORMALDEHYDE** just ***BEFORE*** its use
ZENKER-FORMOL
42
it is an EXCELLENT MICROANATOMIC FIXATIVE for PITUITARY, BONE MARROW, **BLOOD-CONTAINING ORGANS**
ZENKER-FORMOL (Helly's) ***"Bloody helly"***
43
Zenker Formol is aka __________
HELLY'S
44
fixative recommended for TUMOR BIOPSIES especially of the SKIN
HEIDENHAIN'S SUSA
45
Heidenhain's Susa produces minimum shrinkage and hardening of tissues due to the COUNTER-BALANCE of the SWELLING EFFECTS of _____ and the SHRINKAGE effect of _______________
ACIDS; MERCURY
46
fixative used on **WET SMEARS** for cytologic examinations
SCHAUDINN'S SOLUTION
47
commonly used for BONE MARROW BIOPSIES
LILLIE'S B-5 FIXATIVE ("Lillie's Bone Marrow Biopsies") 😂
48
what is added to 10mL Lillie's B-5 fixative prior to use?
1mL of 40% FORMALDEHYDE
49
2 mercuric chloride rapid fixatives giving excellent NUCLEAR preservation
1. CARNOY-LEBRUN 2. OHLMACHER
50
a class of fixatives which are strong oxidizing agents used for PRECIPITATING PROTEINS and PRESERVING CARBOHYDRATES
CHROMATE FIXATIVES
51
recommended for "CAM" (CHROMAFFIN TISSUES, ADRENAL MEDULLA, and MITOCHONDRIA)
CHROMATE FIXATIVES
52
what must be added to chrome-containing tissues before use to prevent counteracting effects and consequent decomposition of solution upon prolonged standing?
FORMALDEHYDE
53
under chromate fixatives: it PRECIPITATES ALL PROTEINS and adequately PRESERVES CARBOHYDRATES
CHROMIC ACID
54
recommended for the demonstration of CHROMATIN, MITOCHONDRIA, MITOTIC FIGURES, GOLGI BODIES, RBC and COLLOID-CONTAINING TISSUES
REGAUD'S FLUID
55
Regaud's Fluid is a fixative with SLOW PENETRATION and tends to produce precipitates of what substance?
SUB-OXIDES
56
prolonged fixation in this fixative **produces MELANIN** and blackens the tissue pigments
REGAUD'S FLUID
57
What is added to Regaud's Fluid just before use?
40% STRONG FORMALDEHYDE
58
fixative recommended for study of **EARLY DEGENERATIVE PROCESSES** and **TISSUE NECROSIS**
ORTH'S FLUID
59
demonstrates **RICKETTSIA** and other bacteria
ORTH'S FLUID
60
Potassium Dichromate is used in how many percent aqueous soln?
3%
61
Potassium Dichromate preserves mitochondria at what pH?
pH 4.5 - 5.2
62
Lead Fixatives are used in how many percent aqueous solution of BASIC LEAD ACETATE?
4%
63
a primary reaction product precipitate for the visualization of the activity of glutamic oxaloacetic transaminase in tissue sections
LEAD OXALOACETATE
64
fixative recommended for **ACID MUCOPOLYSACCHARIDES**
LEAD FIXATIVES
65
disadvantage of lead fixatives: it takes up CO₂ to form what insoluble compound especially on prolonged standing?
LEAD CARBONATE
66
this fixative is a solution of **MERCURIC CHLORIDE, SODIUM CHLORIDE, ALCOHOL, and GLACIAL ACETIC ACID**
SCHAUDINN'S SOLUTION

PSALM GIRLS: HISTOPATHOLOGY (11 decks)

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