EMBEDDING

Question 1

the process by which the impregnated tissue is placed into a precisely arranged position in a mold containing a medium, which is then allowed to solidify

Answer 1

EMBEDDING

Question 2

Embedding is also known as…

Answer 2

CASTING, BLOCKING

Question 3

Paraffin embedded tissues are arranged at the bottom of the mold together with their proper labels and immersed in melted paraffin at what temperature?

Answer 3

5-10°C ABOVE its MELTING POINT

Question 4

the wax is cooled rapidly in a refrigerator at ____ or immersed in __________ to solidify

Answer 4

-5°C; COLD WATER

Question 5

the process by which a tissue is ARRANGED IN A PRECISE POSITION IN THE MOLD DURING EMBEDDING, on the microtome before cutting, and on the slide before staining

Answer 5

ORIENTATION

Question 6

TYPES OF BLOCKING-OUT MOLDS

consists of 2 L-SHAPED PIECES of heavy brass or metal

Answer 6

LEUCKHART’S EMBEDDING MOLDS

Question 7

TYPES OF BLOCKING-OUT MOLDS

made up of INTERLOCKING PLATES resting on a flat metal base, forming SEVERAL COMPARTMENTS

Answer 7

COMPOUND EMBEDDING UNITS

Question 8

TYPES OF BLOCKING-OUT MOLDS

consist of special stainless steel base mold fitted with a PLASTIC EMBEDDING RING which later serves as the block holder during cutting

Answer 8

PLASTIC EMBEDDING RINGS and BASE MOLD

Question 9

TYPES OF BLOCKING-OUT MOLDS

available in 3 different sizes, PEELED OFF ONE AT A TIME

Answer 9

PEEL-AWAYS

Question 10

TYPES OF BLOCKING-OUT MOLDS

used in ordinary refrigerators RECOMMENDED FOR BUSY ROUTINE LABORATORIES

Answer 10

PLASTIC ICE TRAYS

Question 11

TYPES OF BLOCKING-OUT MOLDS

cheap and easy to make; used for EMBEDDING CELLOIDIN and PARAFFIN BLOCKS

Answer 11

PAPER BOATS

Question 12

the process in which tissues are FIRST INFILTRATED WITH CELLOIDIN and SUBSEQUENTLY EMBEDDED IN PARAFFIN MASS

Answer 12

DOUBLE EMBEDDING METHOD

Question 13

in Double Embedding Method, the tissues are first infiltrated with ______ and subsequently embedded in __________ mass

Answer 13

CELLOIDIN; PARAFFIN

C comes before P, wow!

Question 14

this method has SUPERIOR RESULTS FOR LIGHT MICROSCOPIC STUDIES, particularly in hard tissues and for HIGH RESOLUTION LIGHT MICROSCOPY OF TISSUE SECTIONS

Answer 14

PLASTIC / RESIN EMBEDDING

Question 15

PLASTICS are classified into what 3 types?

Answer 15

1. EPOXY PLASTICS
2. POLYESTERS PLASTICS
3. ACRYLIC PLASTICS

“EPA”

Question 16

PLASTICS:

balance mixture of EPOXY PLASTIC, CATALYSTS, and ACCELERATORS

Answer 16

EPOXY PLASTICS

Question 17

PLASTICS:

slow infiltration because of LARGE MOLECULE EPOXY PLASTIC

Answer 17

BISPHENOL A

Question 17

PLASTICS:

have lower viscosity; often sold as MIXTURES OF ISOMERS

Answer 17

GLYCEROL

Question 18

PLASTICS:

can be obtained PURE, have very low viscosity, INFILTRATE FASTEST

Answer 18

CYCLOHEXENE DIOXIDE

Question 19

PLASTICS:

other term ofr BISPHENOL A

Answer 19

ARALDITE

A for ARALDITE 🌟

Question 20

PLASTICS:

other term for GLYCEROL

Answer 20

EPON

Question 21

PLASTICS:

other term for CYCLOHEXENE DIOXIDE

Answer 21

SPURR

Question 22

PLASTICS:

ORIGINALLY INTRODUCED FOR ELECTRON MICROSCOPY in the mid-1950; superseded by more superior oxides and are now seldomly used

Answer 22

POLYESTER PLASTICS

Question 23

PLASTICS:

made up of ESTERS OF ACRYLIC or METHACRYLIC ACID and are used EXTENSIVELY FOR LIGHT MICROSCOPY

Answer 23

ACRYLIC PLASTICS

Question 24

popular embedding medium for LM (EXTREMELY HYDROPHILIC)

Answer 24

POLYGLYCOL METHACRYLATE (GMA)

Question 25

IDEAL embedding medium for UNDECALCIFIED BONE and other HARD TISSUES

Answer 25

METHYL METHACRYLATE (MMA)

Question 26

added to the plastic as a CATALYST that decomposes phenyl radicals

Answer 26

BENZOYL PEROXIDE