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PSALM GIRLS: HISTOPATHOLOGY > DEHYDRATION > Flashcards

DEHYDRATION Flashcards

1
Q

the process of REMOVING INTRACELLULAR & EXTRACELLULAR WATER from the tissue following fixation and prior to wax impregnation

A

DEHYDRATION

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2
Q

IDEAL volume for dehydration

A

10x

NOT LESS THAN 10x the vol of tse

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3
Q

dehydration is best accomplished by the use of ___________ grades of alcohol

A

INCREASING

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4
Q

grades of alcohol to be used

A

70% → 95% → 100%

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5
Q

transfer of tissue directly from formalin to higher grades of alcohol is risky and could lead to ___________ of tissues

A

DISTORTION

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6
Q

CONCENTRATED SOLUTIONS make tissues _______ & _______, making it difficult to cut

A

HARD, BRITTLE

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7
Q

prolonged storage in LOWER CONCENTRATIONS tends to ________ the tissue

A

MACERATE

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8
Q

UNDOUBTEDLY THE BEST DEHYDRATING AGENT

A

ETHANOL

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9
Q

purity of Ethanol to be used

A

99.7%

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10
Q

SUBSTITUTE for good-grade absolute ethyl alcohol

A

ISOPROPANOL

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11
Q

toxic dehydrating agent; for blood and tissue films and for smear preparations

A

METHANOL

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12
Q

utilized for PLANT and ANIMAL MICRO-TECHNIQUES

A

BUTANOL

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13
Q

RECOMMENDED for tissues which DO NOT REQUIRE RAPID PROCESSING

A

BUTANOL

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14
Q

a cheap, rapid dehydrating agent utilized for most URGENT BIOPSIES

A

ACETONE

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15
Q

dehydrating agent with limited use only to small pieces of tissues due to its extreme VOLATILITY & FLAMMABILITY

A

ACETONE

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16
Q

EXCELLENT DEHYDRATING AND CLEARING AGENT miscible to water, melted paraffin, alcohol, and xylol

17
Q

Dioxane is also known as

A

DIETHYLENE DIOXIDE

18
Q

dehydrating agent with the main disadvantage of being expensive, toxic, and extremely dangerous

A

DIOXANE

expensive paminawon si Dioxane

19
Q

dehydrates rapidly and not harmful to tissues

A

CELLUSOLVE

“solve!” kay paspas mag dehyd, di pa jud harmful

20
Q

toxic by inhalation, skin contact, and ingestion

A

CELLUSOLVE

21
Q

other name for Cellusolve

A

ETHYLENE GLYCOL MONOETHYL ETHER

22
Q

used to dehydrate sections and smears following certain stains

A

TRIETHYL PHOSPHATE

23
Q

DEHYDRATES AND CLEARS TISSUES since it is miscible in water and paraffin

A

TETRAHYDROFURAN

24
Q

toxic if ingested or inhaled; vapors cause NAUSEA, DIZZINESS, and HEADACHE

A

TETRAHYDROFURAN

25
Q

it is an EYE and SKIN IRRITANT and prolonged exposure may cause CONJUNCTIVITIS

A

TETRAHYDROFURAN

26
Q

the number of months of exposure to Tetrahydrofuran that causes conjunctival irritation:

27
Q

the temperature that will HASTEN ALCOHOL DEHYDRATION and is used for tissue sections that require URGENT EXAMINATION OF FRAGMENTARY BIOPSIES

28
Q

3 additives that are added to dehydrating agents in DULY HARD TISSUES

A
  1. 4% PHENOL
  2. GLYCEROL-ALCOHOL MIXTURE
  3. MOLLIFLEX

PSALM GIRLS: HISTOPATHOLOGY (11 decks)

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