Tour of the Cell Flashcards
how do biologists study cells
microscopes and biochemistry tools
what do eukaryotic cells have that compartmentalizes their functions
internal membranes
what organelle is probably going to be seen first when examing a cell
the nucleus
where is genetic instruction housed in eukaryotic cells
the nucleus
what carries the genetic instructions in eukaryotic cells
the ribosomes
what is not an organelle in cells
ribosomes
what does the endomembrane system regulate
- protein traffic
- performs metabolic functions
mitochondria and chloroplasts can do what
change energy from one form or another
what does the cytoskeleton do in the cell
organizes structures and activties
extracellular components and connections between cells do what
helps coordinate cellular activties
all organisms are made from
cells
what is the simplest collection of matter that can e alive
cells
all cells are related by
descent from earlier cells
what does being alive mean
able to reproduce itself
cell _____ is correlated to cell ______
structure and function
cell theory
- cells are the fundamental unit of life
- simplest unit capable of independent existent
- living things have to be made from cells
viruses are ______ because they need _______ to survive
not alive and a host cell
why are microscopes used
to visualize cells
why are cells complex
they are self sustaining units
Light microscope
visible light is passed through a specimen and then through glass lenses
how can the image be magnified
the lens refracts the light
three important parts of microscopy
- magnification
- resolution
- contrast
magnification
ratio of an object’s image size to its real size
resolution
the minimum distance of two points that are still distingushable
what is a measure of clarity
resolution
contrast
visible difference in parts of the sample
(light vs dark areas)
what magnification does an LM do to a specimen
about 1000 times actual size
what techniques can make specimen more clear for LM
- fluorene
- stains
- pigments
what is too small to be seen by LM
organelles
unstained specimen of LM has
little contrast
stained specimen of LM has
enhanced contrast
what happens to the stained cell during microscopy
its killed
LM phase-contrast used for
living unpigmented cells
Lm differential-Interference Contrast results in
a 3D image
Fluorescence microscopy of LM uses
antibodies to see specific molecules
what LM advances allow a sharper 3D image
confocal microscopy and deconvolution microscopy
confocal microscopy of LM
laser produces sharper images and allows 3D image reconstruction
Types of electron microscopy (EM)
- scanning electron microscopes (SEM)
- transmission electron microscopes (TEM)
scanning electron microscopes (SEM)
provides a 3D image of the surface of the specimen
scanning electron microscopes (SEM) focus electrons ____ the specimen
onto the surface of
transmission electron microscopes (TEM)
provides a study of the internal structures
transmission electron microscopes (TEM) focus electrons _____ the specimen
through
super-resolution microscopes
Able to “break” the resolution barrier and distinguish structures as small as 10-20 nm across
greater magnification means
additional details cannot be seen clearly
Electron Microscopes
advantage
Revealed many subcellular structures impossible to see with LM
Electron Microscopes
disadvantage
Methods used to prepare the speciment kills the cells
Electron Microscopes
disadvantage
Methods used to prepare the specimen kills the cells
cell fractionation
cells are broken open and separated into their components
______ is used to separate organelles from one another by ______ _____ the centrifugation ____
centrifuges, gradual increasing, speed
the _____ of organelles can be studied when
function and isolated from other components
cells are made from _____ _____
90% and water
two types of cells
prokaryotic and eukaryotic
what two domains of life are fully prokaryotic cells
bacteria and archae
domains in eukaryotic cells
animal, fungi, plant and protist
what 4 features do ALL cells have
- plasma membrane
- cytosol
- chromosomes
- ribosomes
what carries the genes
chromosomes
what makes proteins
ribosomes
PROKARYOTIC CELLS are characterized by
- no nucleus
- DNA is unbound in the nucleoid
- no membrane-bound organelles
- cytoplasm bound by plasma membrane
similarities between PROKARYOTIC and EUKARYOTIC cells
- both have DNA as genetic material
- both are membrane-bound
- both have ribosomes
- both have similar metabolism
differences between PROKARYOTIC and EUKARYOTIC cells
- Eukaryotes have a nucleus AND membrane-bound organelles
- Prokaryotic DNA floats freely while Eukaryotic DNA is held in the nucleus
- Eukaryotic cells are about 10 x bigger
- DNA of eukaryotes is more complex
- Prokaryotes have a cell wall made from peptidoglycan while eukaryotes don’t have cell walls made from that
the cell wall is found in
bacteria, fungi and plants
what makes prokaryotic cell walls unique from all other eukaryotic cell walls
made from peptidoglycan
EUKARYOTIC cells are characterized by
- membrane-bound organelles
- bigger size
- have DNA in nucleus, surrounded by nuclear envelope
- cytoplasm between nucleus and plasma membrane
the plasma membrane is what kind of barrier
selective barrier
what does the plasma membrane allow passage of
- oxygen
- nutrients
- waste
what does the passage of the plasma membrane do for the cell
supports the cell volume
what is the structure of the biological membrane
phospholipid bilayer
what makes species unique from each other
glycoprotein
what is unique to each cell
DNA
how do animal and plant cells differ
from their structure
how is the membrane fixed
from vesicles transporting materials from the ER or golgi
the membrane has of what organelle has no phospholipid membrane
ribosomes
all the organelles in the endomembrane system have
membranes surrounding them
unique structures to plant cell structures
- chloroplasts
- large central vacuoles
- cell walls
- plasmodesmata
how do ribosomes make proteins
by using the information from the DNA
what is the most conspicuous organelle
the nucleus
the ______ encloses the nucleus
nuclear membrane
the nuclear membrane has ____ parts each consisting of a _____
two and lipid bilayer
chromatin
the folding and positioning define what the cell can do
what regulates entry and exit of molecules from the nucleus
pores
what determines the shape of the nucleus
nuclear lamina
what is the nuclear lamina made from
proteins
what are the sections of the DNA called in the nucleus
chromosomes
what is a chromosome made from
single DNA molecule with proteins
what is DNA and proteins on chromosome called
chromatin
nucleolus
site of rRNA synthesis in nucleus
what are ribosomes made from
rRNA and protein
nuclear envelope
encloses the nucleus and separates its contents from the cytoplasm
Nuclear matrix
framework of protein fibers that extend throughout the nuclear interior