Theriogenology Flashcards
What are the collection devices used for semen collection?
Artificial vagina
What is a method of collection for semen collection?
Collection device, digital manipulation, electroejaculation
What are the three parts of ejaculate?
First fraction, second fraction, a third fraction
What is the first fraction?
Clear fluid
What is the second fraction?
Sperm Rich, should be cloudy
What is the third fraction?
Clear fluid, can continue for up to 10 minutes
How do you evaluate semen
Appearance, volume, concentration, motility, percent morphology
What is normal volume of semen in a dog?
Two mills to 20 mills
How can you kill sperm
Formaldehyde
What can you use to measure the concentration of sperm?
Hemocytometer, spectrophotometer
What are the terms used for sperm motility?
Progressively motel, nonmotile, non-progressively motile
What are the techniques used to evaluate sperm’s motility?
Manual motility estimate using wet mount – dilute semen sample with warm extender and place on to slide with cover slip and examine
What is the second technique used to evaluate sperm motility?
Check motility estimates – prepare a wet amount of diluted semen from our photograph using an exposure time of about 0.2 seconds
What is the third technique to evaluate for motility?
Computer aided motility analysis– similar to manual tracking method, but computers do the tracking
What should be the result for motility test
Greater than 70% progressive
What should you examine on a sperm?
Head, mid piece, tail, acrosome, sperm membranes