Sample Handling And Collection Flashcards
What type of tube gives you whole blood?
Red top tube
What tube gives you whole blood with anticoagulant?
Purple top
What is plasma?
Fluid portion of whole blood contains all of the parts of blood
What is serum?
Plasma without fibrinogen
When are anticoagulant required?
Whole blood or plasma is needed
What does heparin interfere with?
Staining of white blood cells
What is EDTA preferred for?
Hematologic studies
What can excessive amounts of EDTA cause?
Cells to shrink and invalidate cell counts in automatic analyzer
What are example examples of oxalates?
Sodium potassium, ammonium, or lithium salts
What is the most commonly used oxalate
Potassium
What are examples of citrates?
Sodium or lithium salts
What is the most common citrate
Sodium
What is sodium fluoride used for?
Glucose preservative
What does sodium fluoride interfere with?
Enzymatic test
What is the normal PVC?
37 to 55%
What is a well hydrated animals PCV
50%
What is a dehydrated animals PCV
70%
What is a 70% PVC symbolize
More cells compared to fluid
What should you do if transferring from syringe to tube?
Remove needle
What does removing the needle prevent?
Least amount of hemolysis as possible
What should you do with tubes containing anticoagulant?
Invert them
What should you label your tubes with?
Date and time, patient name and ID number, owner name
What is the order of draw?
Light blue, red, tiger, top, green, lavender, gray