Common Stains

Question 1

Why do we stain things?

Answer 1

More easily identify cells, Lyson needed cells, look for specific things in cells

Question 2

What stains well with Romanowski stain?

Answer 2

Organisms and cytoplasm, nuclear detail is usually sufficient

Question 3

What does Romanowski poorly stain?

Answer 3

Mass cell granules, basofills, bone marrow cells

Question 4

What is another name for Romanowski stain?

Answer 4

DiF quick

Question 5

What is step one to DiF quick stain?

Answer 5

Fixative – 95% methanol, light blue

Question 6

What is step 2 diff quick stain

Answer 6

Eosin g, acidic ph

Question 7

What is step three to Diff quick stain?

Answer 7

New methylene blue

Question 8

What is the pH of new methylene blue stain?

Answer 8

Alkaline

Question 9

What are two common uses for new methylene blue stain?

Answer 9

Reticulocyte counts, Heinz bodies

Question 10

Which shows better nuclear detail, Romanowski or new methylene blue

Answer 10

New methylene blue

Question 11

What are advantages to papanicolaou stains?

Answer 11

Excellent nuclear and cytoplasmic detail

Question 12

What are disadvantages to papanicolaou stains

Answer 12

Multiple steps and time-consuming, harder to find, prepare, maintain, cells must be wet fixed, use a protein coated slide to prevent cells from lining off when immersed, can be expensive

Question 13

What does it mean for a cell to be wet fixed?

Answer 13

Spray the smear with a cytologic fixative, or place ethanol immediately after prep

Question 14

What stain should you use for an ear cytology?

Answer 14

Diff quick

Question 15

What view should you view an ear cytology under?

Answer 15

Oil of immersion

Question 16

What are normal findings on an ear cytology?

Answer 16

Scream, more epithelial cells, a few organisms, cerumen

Question 17

What are abnormal findings in an ear cytology

Answer 17

Mites, cocci, rods, yeast

Question 18

What is another name for yeast?

Answer 18

Malassezia