test 2: HB and Mb part 2 Flashcards
what is a protein family
evolutionaryily related proteins
globin fam
what does a hyperbola mean of cooperativity and sigmoidal
hyperbola = no cooperativity
sigmoidal = coopeativiity
what is the allosteric effect
binding at one site affects binding at others
how many subunits are required for allosterism
more than 1 subunit
what does the S curve indicate
allosterism
what is HOMOtropic allosteric interaction
effector and ligand are regulated by the same molecule
O2 and Hb
what is heterotropic allosteric interaction
effector and liganrd are differnt
what is an activator and what is an inhibitor
- activator shifts the eq toward R state= positive interaction
inhibitor shifts towards the T state meaning - interaction
descrube Hb oxugen affinity
starts out high but then FAVORS T state in the eq between T and R
what does BPg, h+ and co2 favor
tense low affinity
what are BPG,H+ and Co2 involved in
the Bohr effect
what does O2 favot
relaxed state
what is the bohr effect
- increased temp = release of O2 from Hb
- increase Co2, increase H+, increasing temp = release of O2 = decrease in affinity of H2
what happens to the curve if the affnity is decreased
curve shifts to the right
what happens withn
Temp increase
ph increase (basic)
co2 decrease
curve shifts to the left
how does temperature increase affect Hb affinity
high temp like fever will cause Yo2 to decrease wh
where on T state do Bohr protons bind
- N terminal amino gprps and His 146 betal
what amino group is responsiblel for 2-30% of the bohr effect
N terminal amino groups
what happens to the pka during transition
changes the pka causing them to release coordinated H
how much does his 146 account for bohr effect
40
what protons ar released when Hb binds O2
H+ protons
where on the T state does CO2 bind
-N terminal amino groups and it can form carbamates
- not present in the R state