etc part 1+ ox phos part 1 Flashcards
where does etc and ox phos happen in euk.
inner mito membrane
where does oxphos and etc happen in prok
inner cell membrane
what energizes the etc
nadh and fadh2 made in glyc. pdh rxn and tca
where is more atp made
by ox phos
what is inner membrane impermeable to
- most ions and molecules like H+
where are the high energy electron paris NADH2 and FADH2 transferred to
oxygen via etc
how is energy released in the mito used
- used to pump protons into the intermembrane space to get charge separation
what is the mitochon known for
cellular batter
what drives atp synthase
electrochemical energy of protons falling back to matrix
what is the route of NADH in etc
Nadh > I> Q> 3> cyt c> 4> o2
produces 10H+
route of fadh2
fadh2>2>Q>3>cytc > 4> o2
6H+ out
what are the moble e- carriers
ubiqunione and cytochrome c
what is proton motive force
concentration gradient of H= across a membrane is harnessed to make atp
what complex has fmn redox coenzyme
1
coenzyme q is present in what complex
3
what does ubiquionene do
carries e= from complex I/II to complex 3
what complex has the rieske iron sulfer center and q cycle
complex 3
where does cytochrome c carry electrons
complex 3 to 4
why us cytochrom c diff
peripheral membrane protein while other cytochromes are integral
1 electron carrier needs how many cyt cs
2 cyt c ( 2 electrons from nadh)
what is a protein w/ a heme posthetic group that is involved in e- transfer
cytochromes
what does the heme fe alternate between
Fe+2 (ferrous) and Fe+3( ferric) states
what happens to the coordination sites for the hemes in cytochrom
- all 6 are occupied do not bind to O2
what atom does complex 4 have
copper
cycle between reduced Cu+ (cuprous) and oxidized Cu+2 state cupric
what cytochrome does complex 4 have
cyt a3 and binds and reduces O2 to water
what complex has cytochrome bc1 comples, coQ cytochrom c reductase, cytochrome c-CoQ oxidreductase
3
wht complex has cytochrome c oxidase
4
succinate dh is what complex of etc
2
what does complex 2 do
transfers e- directly to q skipping complex 1
- doesn’t directly contribute to pumpoing out of protons
how did scientists disocer the order of the sequence of complexes
measured standard reduction potentials of all components - more positive the closer to O3
how did scientists discover thre order of the sequence of complexes
- look for intercting between complexes
- inhibitors see which metabolites build up
what happens with o2 consumption
stops an addition of an inhibitor
