etc part 2 and ox phos Flashcards
what are the energy sources of chemiosmosis
active transport
electron potential
flagellar rotation
atp p
nadh synthesis
heat produ
what are the components of atp synthase
3 ab dimers
c subunit
which moves on atp synthase
c rotor and y subunit rotate not the 3 ab dimers
what do the ab dimers undergo
conformational changes adp + pi = atp
in atp synthase when h+ is low what happens
hydrolyze atp and moves in reverse
how does H+ move in atp sythase
high to low and reverse low to high
outer membrane is permeable to what of the mito
small molecules and ion
inner membrane of mito impermeable to waht
most small molecules and ions including H=
what does the inner membrane mito contain
respiratory electron carriers complex 1-4
-adp-atp translocases
-atp synthase
- membrane
what is the adenine nucleotide translocase antiport
moves 4 negative charges out for 3 negative charge in adp
what is phosphate translocase symport
movement of H2Po4 inward is not favored
atp synthesis costs
3H+ for atp synthease
-1 pi for translocation
what is the phos ox ration or p/o ration
measure of mlcls of atp made per e- pair carried through electron transport
how many atp made per 2e
4 H+ flow back into matrix per atp
3 for atp synthesis
1 for pi
10/4 = 2.5 per nadh
6/4 = 1.5 atp per fadh2
where is the nadh made in glycolysis
in the cytosol and it can’t cross in to give e- to etc
what is a shuttle
pass electrons to something that can penetrate the mito membrane
1. insect brain and flight muscle
2. mammalian muscle and liver
what is an example of a shuttle
glycerol-PO4 shuttle, malate aspartate shuttle
what is the malate aspartate shuttle
- malate is transported into the mito and can be reoxid to oxalocaetate to make nadh for etc
descrube fast twitch muscles
- short burts of energy
- anaerobic glycolyis for atp
describe slow twitch muscles
- prolonged energy
- rich in mitos
- obtain atp from ox phos
- glifht muscles of birds and leg runners
dark meat vs white meat
white uses fast twitch mscucles
dark uses slow twitch and is rich in mitos
are etc and ox phos coupled
yes and if etc stops, ox phos stops
what does uncoupling cause
disrupting the H+ gradient
what is thermogenin
found in mito
heat producing brown fat
what do UCP do (uncouple protein)
channel that permit import of H+ from intermembrane space to matrix WITHOUT atp synthesis
what is non shivering thermgeneis
used to maintain body tem in hibernating animals
what is a cpd that is hydrophobic enough after binding H+ to cross the membrane
2,4-dinitrophenol
when does atp synthase stops
when FCCP interacts
what is an atp synthase inhibitor
oligomycin A
what does oligomycin A do
- antibiotic
binds F0 complex
blocks H_ flow thorugh F0 channel
