slideshow 8

Question 1

E cell genetic material

Answer 1

yes dna
multiple linear chrs that are in a nucleus and contain histone proteins

Question 2

P cell genetic material

Answer 2

yes DNA
a single circular chromosome
naked dna

Question 3

E cell Meiosis

Answer 3

YES

Question 4

P cell Meiosis

Answer 4

no. does binary fission. clones of previous cell

Question 5

E cell recombination

Answer 5

yes . independent assortment or crossing over

Question 6

P cell recombination

Answer 6

yes

Question 7

do E or P cells do crossing over >

Answer 7

both

Question 8

Bacteria colony is what

Answer 8

cell clones descended from a singly original cell

Question 9

commonly studied genetic mutants in bacteria

Answer 9

auxotrophic alleles, energy use mutant alleles, antibiotic resistant alleles

Question 10

auxotropic alleles def (pur-)

Answer 10

unable to synthesize a molecule required for life. in this case purines (A+G)

Question 11

Energy use mutant allels (lac-)

Answer 11

the mutant bacterium cannot use a specific nutritive molecule to produce ATP in this case, cant use lactose to produce ATP

Question 12

Antibiotic resistant alleles (str s)

Answer 12

str s means with cell will perish when exposed to the antibiotic streptomycin. s is sensitive . r is resistant

Question 13

when adding lactose only we what

Answer 13

are selecting against the lac - bacteria

Question 14

how can we harvest and study lac - mutants

Answer 14

with replica plating

Question 15

in lac- and lac+ replicate plating we make the two dishes with what

Answer 15

one of glucose and lactose and one of lactose only

Question 16

its easier to harvest what

Answer 16

antibiotic resistant mutants, because they are the ony ones that will survie

Question 17

with an auxotrophic mutant we start with histidine but then what to select for his -

Answer 17

one gets no histidine and one still adds histidine

Question 18

with energy use mutants we start with lactose and glucose but when what to select for lac-

Answer 18

one we only add lactose and the other we add lactose and glucose

Question 19

with antibiotic resistance mutants we start plain. how do we select or str R

Answer 19

we add streptomycin

Question 20

if met- bio- thr+ leu+ thi+ and met+ bio+ thr- leu- thi- mix what could form colonies ?

Answer 20

1/1000 are
met+ bio+ thr+ leu+ thi+

Question 21

what colonies are formed from the met bio thr leu thi test

Answer 21

prototrophic colonies (all were +)

Question 22

can bacteria share DNA

Answer 22

yes

Question 23

MM is what

Answer 23

a minimal medium plate

Question 24

Donor and REcipient means

Answer 24

they are names for cells. donor gives dna. recipient recieves it.

Question 25

in donor and recipient how do they switch the dna

Answer 25

if they cross over at just the right points

Question 26

can dissolved compounds pass through the filter of the U tube

Answer 26

yes

Question 27

can bacterial cells pass throught the filter of the U tube

Answer 27

no

Question 28

Can EColi recomine ? if yes , how?

Answer 28

yes , it requires physical contact

Question 29

can bacteria share dna

Answer 29

yes

Question 30

main diff of horizontal gene transfer

Answer 30

all the cells are apart of the same generation

Question 31

what is it if a donor gives his + to another cell. it links up with a his - and switches dna

Answer 31

becomes a new his+ cell. this is horizontal gene transfer

Question 32

3 forms of horizontal gene transfer

Answer 32

conjugation
transformation
transduction

Question 33

conjugation def

Answer 33

uses a physical bridge between bacteria to transfer DNA

Question 34

transformation def

Answer 34

involves recipient cells picking up bacterial DNA in the environment, sourced from deaaad donor cells

Question 35

transduction def

Answer 35

the viruses of bacteria accidentally transfer dna from donor to recipient cells

Question 36

3 types of ecoli cells

Answer 36

F+ cells, F- cells, and Hfr cells

Question 37

F= cells def

Answer 37

carry an accessory, circular piece of DNA called an F- plasmid

Question 38

what does and F-plasmid do

Answer 38

creates little stringy pili to form around the bacteria cell

Question 39

F- cells def

Answer 39

they lack an F plasmid

Question 40

Hfr means

Answer 40

High frequency recombination

Question 41

Hfr def

Answer 41

cells possess an F-plasmid too, but it has been integrated into the bacterial chromosome.

Question 42

What can an F+ cell become

Answer 42

Hfr cell

Question 43

spontaneous integration def

Answer 43

the moment it switches from f+ to Hfr

Question 44

Conjugative pilus def

Answer 44

is the bridge which DNA crosses from donor (F + or Hfr) to the recipient (F- cell)

Question 45

3 points when the F+ donates to F-

Answer 45

F- will become an F+
no recombination occurs
conjugative pilas will break

Question 46

integration of the f plasmid can be 1 or 2 ways

Answer 46

2. it can be flipped

Question 47

3 points when the Hfr donates to F-

Answer 47

F- is still an F-
F- gets his+ now (or whatever)
allows for recomination to POSSIBLY occur
conjugative pilas will break

Question 48

nucleases def

Answer 48

the thing that eats up the f plasmid dna in the f- cell

Question 49

Exogenote def

Answer 49

dna from donor cell

Question 50

endogenote def

Answer 50

recipient cells chromosome