Slide Preparation Technique

Question 1

Intact parts are directly mounted on the slide

Answer 1

whole mount

Question 2

Examples of Whole Mount

Answer 2

small intacts, embryos, plant parts

Question 3

to separate individual cells that are bonded tightly or clustered together

Answer 3

macerations

Question 4

how to perform maceration

Answer 4

use strong acids to hydrolize

Question 5

thin film of cells

Answer 5

smear

Question 6

used on large specimens that are too big for whole mounting and too valuable for maceration/squashing

Answer 6

section (thin)

Question 7

preparation excellent for chromosome studies

Answer 7

squash

Question 8

steps in squash (3)

Answer 8

1. immerse tissue in chemicals to preserve and soften
2. use a bent needle to tease apart
3. place cover slip then pressure to flatten cells

Question 9

steps for slide preparation (5)

Answer 9

1. collection
2. fixation
3. staining
4. mounting
5. storage

Question 10

chemicals used for fixation

Answer 10

fixatives

Question 11

what to do after 24 hrs immersed in fixative

Answer 11

stored in mild chemical solution

Question 12

types of basic stain

Answer 12

acetocarmine

Question 13

types of negative stain

Answer 13

eosin/fast green

Question 14

2 steps under differential staining

Answer 14

primary stain then counterstain

Question 15

where to mount specimen

Answer 15

particular medium of high-refractive index

Question 16

where to mount those LABILE in alcohol and non=polar solvents

Answer 16

glycerin jelly / hoyer’s medium

Question 17

most mounting are what based

Answer 17

oil-based and non-polar which requires dehydrated specimen

Question 18

what to include in storage

Answer 18

name of specimen,
description of materials,
date of preparation,
method of slide preparation
name of worker