Microscope Flashcards
who did the earliest records of microscopic observations
anton van leeuwenhoek
what did anton van leeuwenhoek first observe and what type of microscope did he use
protozoans - simple microscope
how did early microscopes first start out and where
lenses in tubes - holland
Magnification vs Linear Magnification
Magnification: magnitude of amplification of image by the microscope
Linear Magnification: magnitude of amplification of image by the objectives lens only
2 types of magnification
Theoretical (ocular lens x objective lens)
Actual (apparent size/actual size)
Resolving Power vs Limit of resolution
Resolving Power - ability of a microscope to distinguish between two adjacent points as DISTINCT and SEPARATE
Limit of Resolution - smallest distance that can be distinguished by microscope
An __ of the ___-gathering ___ of the ___ lens
index
light
power
objective
NUMERICAL APERTURE
- RELATIONSHIP between NA and LIMIT OF RESOLUTION
- NA of each objective lens
- inversely proportional
LPO - 0.25
HPO - 0.65
OIO - 1.30
The ability of medium to deflect light
and what does a high value of it indicates
refractive index
High RI - less deflection - more light
RI of water and air
air - 1.00
water - 1.56
Working distance vs Field of view
+ value per objective lens
WD - distance between the specimen and objective lens
LPO: 5
HPO: 0.46
OIO: 0.13
FV - area visible under microscope
LPO: 1.50
HPO. 0.35
OIO: 0.18
___ OF FOCUS definition
depth
- property that indicates how deep the space the microscope can bring into clear view
APERTURE DISC VS EXTENSION TUBE
AD- under stage and contains holes w/ diff sizes to regulate light
ET - below eyepiece and also contains lens
How many inches should a microscope be placed far from a table’s edge
2 inches
what to use to clean the lenses
lens paper
