skills 12 Flashcards

1
Q

Indole, Methyl Red, Voges-Proskauer, Citrate (IMViC) test

{purpose}

A
  • block of tests done together
  • investigates aspects of bacterial metabolism
  • important in analysis of water (checks for presence of Escherichia ➡ fecal contamination / likelihood of pathogens)
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2
Q

Indole test of IMViC

{purpose}

A
  • detect formation of indole

- differentiate members of family of Enterobacteriaceae (especially E. coli from Enterobacter & Klebsiella)

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3
Q

Indole test (of IMViC) test results

A
  • positive= layer of Kovac’s reagent will almost immediately turn red (rosindole)
  • negative= green; green if reagent gets mixed up (failed test)
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4
Q

Indole test (of IMViC) test products

A

indole, pyruvic acid & ammonia

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